Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2602782..2603401 Replicon chromosome
Accession NZ_CP063867
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISD32_RS12560 Protein ID WP_002892050.1
Coordinates 2603183..2603401 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISD32_RS12555 Protein ID WP_008805436.1
Coordinates 2602782..2603156 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS12545 2597937..2599130 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISD32_RS12550 2599153..2602299 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISD32_RS12555 2602782..2603156 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISD32_RS12560 2603183..2603401 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISD32_RS12565 2603560..2604126 + 567 WP_087731501.1 maltose O-acetyltransferase -
ISD32_RS12570 2604098..2604223 - 126 Protein_2475 hypothetical protein -
ISD32_RS12575 2604263..2604733 + 471 WP_008805434.1 YlaC family protein -
ISD32_RS12580 2604702..2606159 - 1458 WP_110224264.1 PLP-dependent aminotransferase family protein -
ISD32_RS12585 2606260..2606958 + 699 WP_023297138.1 GNAT family N-acetyltransferase -
ISD32_RS12590 2606955..2607095 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISD32_RS12595 2607095..2607358 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179081 WP_002892050.1 NZ_CP063867:2603183-2603401 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179081 NZ_CP084529:4083231-4083485 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179081 WP_008805436.1 NZ_CP063867:2602782-2603156 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179081 NZ_CP084529:4082983-4083234 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References