179071

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 199177..199767 Replicon chromosome
Accession NZ_CP063867
Organism Klebsiella variicola strain M142-3

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISD32_RS00930 Protein ID WP_008804165.1
Coordinates 199435..199767 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISD32_RS00925 Protein ID WP_012541132.1
Coordinates 199177..199434 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISD32_RS00905 194785..195360 + 576 WP_012541129.1 hypothetical protein -
ISD32_RS00910 195539..196375 + 837 WP_008804155.1 alpha/beta hydrolase -
ISD32_RS00915 196582..197553 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISD32_RS00920 197550..198650 - 1101 WP_110224852.1 phosphotransferase -
ISD32_RS00925 199177..199434 + 258 WP_012541132.1 antitoxin Antitoxin
ISD32_RS00930 199435..199767 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISD32_RS00940 200090..201526 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISD32_RS00950 201899..203353 - 1455 WP_008804170.1 AMP nucleosidase -
ISD32_RS00955 203484..203729 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179071 WP_008804165.1 NZ_CP063867:199435-199767 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179071 NZ_CP084529:c2483427-2483320 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179071 WP_012541132.1 NZ_CP063867:199177-199434 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179071 NZ_CP084529:2483475-2483541 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References