Detailed information of TA system TA179070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 37961..38483
• Replicon: plasmid pS130-1
• Accession: NZ_CP063866
• Organism: Klebsiella pneumoniae strain S130-1

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: IOD48_RS25890
• Protein ID: WP_004187019.1
• Coordinates: 38199..38483 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: IOD48_RS25885
• Protein ID: WP_004187025.1
• Coordinates: 37961..38209 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD48_RS25855	35328..35897	-	570	WP_004152117.1	small heat shock protein sHSP20	-
IOD48_RS25860	35932..36213	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
IOD48_RS25865	36457..36720	-	264	WP_004118208.1	hypothetical protein	-
IOD48_RS25870	36735..36998	+	264	WP_004187027.1	transposase	-
IOD48_RS25875	36943..37059	+	117	Protein_41	transposase domain-containing protein	-
IOD48_RS25880	37247..37671	+	425	Protein_42	IS1 family transposase	-
IOD48_RS25885	37961..38209	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
IOD48_RS25890	38199..38483	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD48_RS25895	38499..39854	-	1356	WP_004187017.1	ISNCY family transposase	-
IOD48_RS25900	39936..40235	-	300	Protein_46	conjugal transfer protein TraH	-
IOD48_RS25905	40233..40716	-	484	Protein_47	hypothetical protein	-
IOD48_RS25910	40733..41257	-	525	Protein_48	hypothetical protein	-
IOD48_RS25915	42800..43264	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A)	mrkA / mrkB / mrkC / mrkF / mrkJ	1..150355	150355
	-	flank	IS/Tn	-	-	37441..37707	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T179070 WP_004187019.1 NZ_CP063866:38199-38483 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T179070 NZ_CP084529:2358102-2358437 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT179070 WP_004187025.1 NZ_CP063866:37961-38209 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT179070 NZ_CP084529:2357854-2358102 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4