Detailed information of TA system TA179069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 24321..25057
• Replicon: plasmid pS130-1
• Accession: NZ_CP063866
• Organism: Klebsiella pneumoniae strain S130-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: IOD48_RS25800
• Protein ID: WP_004187044.1
• Coordinates: 24321..24803 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IOD48_RS25805
• Protein ID: WP_003026799.1
• Coordinates: 24791..25057 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD48_RS25775	20345..21281	-	937	Protein_21	ISNCY family transposase	-
IOD48_RS25780	21391..21561	+	171	Protein_22	transposase	-
IOD48_RS25785	21559..21666	+	108	Protein_23	IS3 family transposase	-
IOD48_RS25790	21900..22604	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
IOD48_RS25795	22764..24110	-	1347	WP_200991514.1	ISNCY family transposase	-
IOD48_RS25800	24321..24803	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
IOD48_RS25805	24791..25057	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IOD48_RS25810	25208..25912	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
IOD48_RS25815	26071..28656	-	2586	WP_004187040.1	EAL domain-containing protein	-
IOD48_RS25820	28625..28870	-	246	WP_032238678.1	hypothetical protein	-
IOD48_RS25825	28928..29908	-	981	Protein_31	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A)	mrkA / mrkB / mrkC / mrkF / mrkJ	1..150355	150355
	-	inside	IScluster/Tn	-	-	20324..29908	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T179069 WP_004187044.1 NZ_CP063866:c24803-24321 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T179069 NZ_CP084529:2306198-2306548 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179069 WP_003026799.1 NZ_CP063866:c25057-24791 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179069 NZ_CP084529:2305953-2306204 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66