Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3248719..3249344 Replicon chromosome
Accession NZ_CP063865
Organism Klebsiella pneumoniae strain S130-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag IOD48_RS16175 Protein ID WP_004187928.1
Coordinates 3248961..3249344 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD48_RS16170 Protein ID WP_004150355.1
Coordinates 3248719..3248961 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD48_RS16145 3244721..3245320 + 600 WP_004151865.1 glucose-1-phosphatase -
IOD48_RS16150 3245314..3246174 + 861 WP_004146232.1 virulence factor BrkB family protein -
IOD48_RS16155 3246171..3246608 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD48_RS16160 3246653..3247594 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IOD48_RS16165 3247608..3248525 - 918 WP_004187929.1 alpha/beta hydrolase -
IOD48_RS16170 3248719..3248961 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD48_RS16175 3248961..3249344 + 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD48_RS16180 3249518..3250447 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD48_RS16185 3250444..3251079 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD48_RS16190 3251076..3251978 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T179062 WP_004187928.1 NZ_CP063865:3248961-3249344 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179062 NZ_CP084529:339959-340062 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179062 WP_004150355.1 NZ_CP063865:3248719-3248961 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179062 NZ_CP084529:c340100-339829 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References