Detailed information of TA system TA179048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 81207..81943
• Replicon: plasmid pM63-1
• Accession: NZ_CP063864
• Organism: Klebsiella pneumoniae strain M63-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IOD47_RS26035
• Protein ID: WP_032432065.1
• Coordinates: 81207..81689 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IOD47_RS26040
• Protein ID: WP_003026799.1
• Coordinates: 81677..81943 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD47_RS26020	77920..78860	-	941	Protein_61	ISNCY family transposase	-
IOD47_RS26025	78967..79326	-	360	WP_019704573.1	hypothetical protein	-
IOD47_RS26030	79592..80992	-	1401	WP_094284712.1	ISNCY family transposase	-
IOD47_RS26035	81207..81689	-	483	WP_032432065.1	GNAT family N-acetyltransferase	Toxin
IOD47_RS26040	81677..81943	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IOD47_RS26045	82213..83115	+	903	WP_200995631.1	hypothetical protein	-
IOD47_RS26050	83270..83557	+	288	WP_180247945.1	chorismate mutase	-
IOD47_RS26055	83976..85007	-	1032	WP_180247946.1	GlxA family transcriptional regulator	-
IOD47_RS26060	85249..86700	+	1452	WP_180247947.1	L-tyrosine/L-tryptophan isonitrile synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..161463	161463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17268.93 Da
• Isoelectric Point: 8.7400

>T179048 WP_032432065.1 NZ_CP063864:c81689-81207 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T179048 NZ_CP084521:c871578-871234 [Edwardsiella ictaluri]
ATGAAGTCACTATATTGGGTTGGTAGTACAAAAAAAGACCTCCAGTCATTGCCTGAAGAAGTACAGGATATTTTCGGCTA
TGCCCTGCATCTGGCACAGGTAGGAGGTAAACACTCACAGACCAAGCCACTGAAGGGGTTTAGTGGAGCTGGCGTACTTG
AGGTAGTAGAAGACTTTCTCGGTGATACTTATCGTGCAGTGTATACCGTTAAGTTCGGTGATGCCGTCTACGTTTCGCAC
GTATTCCAGAAAAAGTCTTCATCGGGAATCGCCACGCCAAAGCTCAATATGGACAAGATCCGCGAACGGCTAAAAGCCGC
AGAAAACCATGCCAGAGGAGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179048 WP_003026799.1 NZ_CP063864:c81943-81677 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179048 NZ_CP084521:c871232-870915 [Edwardsiella ictaluri]
ATGAGCCATGATATTGAAGTCAGCAGCGGCAACGTTTATGTCGATATTGGCAGAGATGATGCCGAGGAAATGCTGTTAAA
AGCTCAGCTTGCCACCACTATTGGAAACATCATTAAAAGCCGTAGCCTGACGCAAGAACAAGCAGCAAAACTGCTGGGGA
TGACGCAGCCCAAACTGTCCAATATGCTGCGTGGGCAGTTCCGGGGCATCAGTGAAGCCAAAATGCTGGAATGTCTGACC
CGCTTAGGGCGCGACGTGCAAATCGTGGTTGGAAAGCCGCGCCGGACGCCGGGTAGCGTGAAAGTAGTGTTAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66