Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1468692..1469317 Replicon chromosome
Accession NZ_CP063863
Organism Klebsiella pneumoniae strain M63-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag IOD47_RS06870 Protein ID WP_019705794.1
Coordinates 1468692..1469075 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD47_RS06875 Protein ID WP_004150355.1
Coordinates 1469075..1469317 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD47_RS06855 1466058..1466960 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IOD47_RS06860 1466957..1467592 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD47_RS06865 1467589..1468518 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD47_RS06870 1468692..1469075 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD47_RS06875 1469075..1469317 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD47_RS06880 1469522..1470439 + 918 WP_012737090.1 alpha/beta hydrolase -
IOD47_RS06885 1470453..1471394 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
IOD47_RS06890 1471439..1471876 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD47_RS06895 1471873..1472733 - 861 WP_004146232.1 virulence factor BrkB family protein -
IOD47_RS06900 1472727..1473326 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T179034 WP_019705794.1 NZ_CP063863:c1469075-1468692 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179034 NZ_CP084503:c135967-135689 [Klebsiella pneumoniae]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179034 WP_004150355.1 NZ_CP063863:c1469317-1469075 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179034 NZ_CP084503:c136236-135967 [Klebsiella pneumoniae]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGTGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGCGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References