Detailed information of TA system TA179021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 22848..23491
• Replicon: plasmid pM1026-3Ar.1
• Accession: NZ_CP063859
• Organism: Klebsiella pneumoniae strain M1026-3Ar

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IOD46_RS25075
• Protein ID: WP_016236302.1
• Coordinates: 23075..23491 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: IOD46_RS25070
• Protein ID: WP_001261282.1
• Coordinates: 22848..23078 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD46_RS25040	17857..19032	+	1176	Protein_19	IS3 family transposase	-
IOD46_RS25045	19414..20199	-	786	WP_050485696.1	site-specific integrase	-
IOD46_RS25050	20245..20766	-	522	WP_032448294.1	hypothetical protein	-
IOD46_RS25055	20824..21216	-	393	WP_074422814.1	hypothetical protein	-
IOD46_RS25060	21325..22269	-	945	WP_032448293.1	hypothetical protein	-
IOD46_RS25065	22469..22891	-	423	WP_050485703.1	hypothetical protein	-
IOD46_RS25070	22848..23078	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IOD46_RS25075	23075..23491	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
IOD46_RS25080	23565..25127	+	1563	WP_004206609.1	AAA family ATPase	-
IOD46_RS25085	25112..26134	+	1023	WP_032439672.1	helicase UvrD	-
IOD46_RS25090	26673..27587	+	915	WP_153378766.1	HNH endonuclease	-
IOD46_RS25095	27773..28123	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / catA2 / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B)	iutA / iucD / iucC / iucB / iucA	1..262519	262519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T179021 WP_016236302.1 NZ_CP063859:23075-23491 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T179021 NZ_CP084501:931-1197 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT179021 WP_001261282.1 NZ_CP063859:22848-23078 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT179021 NZ_CP084501:723-947 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3