Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3493084..3493894 Replicon chromosome
Accession NZ_CP063858
Organism Klebsiella pneumoniae strain M1026-3Ar

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag IOD46_RS17095 Protein ID WP_004178461.1
Coordinates 3493361..3493894 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IOD46_RS17090 Protein ID WP_002887278.1
Coordinates 3493084..3493350 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD46_RS17070 3488955..3489299 - 345 WP_064152125.1 cation efflux system protein CusF -
IOD46_RS17075 3489317..3490702 - 1386 WP_064152126.1 efflux transporter outer membrane subunit -
IOD46_RS17080 3490875..3491558 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
IOD46_RS17085 3491548..3492981 + 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
IOD46_RS17090 3493084..3493350 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IOD46_RS17095 3493361..3493894 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
IOD46_RS17100 3493942..3495063 - 1122 WP_004186850.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179019 WP_004178461.1 NZ_CP063858:3493361-3493894 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179019 NZ_CP084498:162941-163090 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179019 WP_002887278.1 NZ_CP063858:3493084-3493350 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179019 NZ_CP084498:c162896-162837 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References