Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33657..34182 Replicon plasmid pN1059-5At
Accession NZ_CP063857
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag IOD44_RS25345 Protein ID WP_013023785.1
Coordinates 33877..34182 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag IOD44_RS25340 Protein ID WP_001568025.1
Coordinates 33657..33875 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS25320 28813..29592 + 780 WP_013023780.1 hypothetical protein -
IOD44_RS25325 29799..31391 + 1593 WP_200973353.1 hypothetical protein -
IOD44_RS25330 31425..32552 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
IOD44_RS25335 32549..32839 + 291 WP_013023783.1 hypothetical protein -
IOD44_RS25340 33657..33875 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IOD44_RS25345 33877..34182 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
IOD44_RS25350 34351..34746 + 396 WP_017899885.1 hypothetical protein -
IOD44_RS25355 34773..35087 + 315 WP_053389906.1 hypothetical protein -
IOD44_RS25360 35098..36114 + 1017 WP_017899884.1 hypothetical protein -
IOD44_RS25365 36312..37106 + 795 WP_004197635.1 site-specific integrase -
IOD44_RS25370 37562..37741 - 180 WP_006788217.1 hypothetical protein -
IOD44_RS25375 37861..38487 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / qnrS1 / blaCTX-M-3 / blaTEM-1B / floR / tet(A) / mph(A) - 1..92970 92970


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T179004 WP_013023785.1 NZ_CP063857:33877-34182 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179004 NZ_CP084494:151733-151882 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT179004 WP_001568025.1 NZ_CP063857:33657-33875 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179004 NZ_CP084494:c151688-151629 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References