Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3766822..3767447 Replicon chromosome
Accession NZ_CP063856
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IOD44_RS18055 Protein ID WP_002882817.1
Coordinates 3766822..3767205 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IOD44_RS18060 Protein ID WP_004150355.1
Coordinates 3767205..3767447 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS18040 3764188..3765090 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IOD44_RS18045 3765087..3765722 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IOD44_RS18050 3765719..3766648 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IOD44_RS18055 3766822..3767205 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IOD44_RS18060 3767205..3767447 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IOD44_RS18065 3767652..3768569 + 918 WP_009309725.1 alpha/beta hydrolase -
IOD44_RS18070 3768583..3769524 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IOD44_RS18075 3769569..3770006 - 438 WP_101986966.1 D-tyrosyl-tRNA(Tyr) deacylase -
IOD44_RS18080 3770003..3770863 - 861 WP_004146232.1 virulence factor BrkB family protein -
IOD44_RS18085 3770857..3771456 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179000 WP_002882817.1 NZ_CP063856:c3767205-3766822 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179000 NZ_CP084493:32118-32405 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179000 WP_004150355.1 NZ_CP063856:c3767447-3767205 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179000 NZ_CP084493:31879-32118 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References