Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2556008..2556627 Replicon chromosome
Accession NZ_CP063856
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IOD44_RS12385 Protein ID WP_002892050.1
Coordinates 2556409..2556627 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag IOD44_RS12380 Protein ID WP_002892066.1
Coordinates 2556008..2556382 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS12370 2551160..2552353 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IOD44_RS12375 2552376..2555522 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
IOD44_RS12380 2556008..2556382 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
IOD44_RS12385 2556409..2556627 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
IOD44_RS12390 2556786..2557352 + 567 WP_002892030.1 maltose O-acetyltransferase -
IOD44_RS12395 2557324..2557464 - 141 WP_004147370.1 hypothetical protein -
IOD44_RS12400 2557485..2557955 + 471 WP_002892026.1 YlaC family protein -
IOD44_RS12405 2557930..2559381 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
IOD44_RS12410 2559482..2560180 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
IOD44_RS12415 2560177..2560317 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IOD44_RS12420 2560317..2560580 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178996 WP_002892050.1 NZ_CP063856:2556409-2556627 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T178996 NZ_CP084492:c4818135-4817851 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT178996 WP_002892066.1 NZ_CP063856:2556008-2556382 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT178996 NZ_CP084492:c4818367-4818125 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References