Detailed information of TA system TA178994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1246173..1247761
• Replicon: chromosome
• Accession: NZ_CP063856
• Organism: Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486RAG1
• Locus tag: IOD44_RS06195
• Protein ID: WP_016946382.1
• Coordinates: 1246173..1247495 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IOD44_RS06200
• Protein ID: WP_024622638.1
• Coordinates: 1247495..1247761 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD44_RS06180	1241229..1243730	+	2502	WP_101987464.1	fimbrial biogenesis outer membrane usher protein	-
IOD44_RS06185	1243718..1244722	+	1005	WP_004184029.1	type 1 fimbrial protein	-
IOD44_RS06190	1244864..1246156	+	1293	WP_040222216.1	glycoside hydrolase family 10 protein	-
IOD44_RS06195	1246173..1247495	-	1323	WP_016946382.1	type II toxin-antitoxin system HipA family toxin	Toxin
IOD44_RS06200	1247495..1247761	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IOD44_RS06205	1248027..1248353	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
IOD44_RS06210	1248350..1248850	-	501	WP_101987253.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
IOD44_RS06215	1248828..1250207	-	1380	WP_101987254.1	purine permease	-
IOD44_RS06220	1250554..1251708	+	1155	WP_023341545.1	FAD-dependent urate hydroxylase HpxO	-
IOD44_RS06225	1251689..1252612	-	924	WP_080882515.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49125.44 Da
• Isoelectric Point: 7.5358

>T178994 WP_016946382.1 NZ_CP063856:c1247495-1246173 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T178994 NZ_CP084492:4101005-4101223 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT178994 WP_024622638.1 NZ_CP063856:c1247761-1247495 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT178994 NZ_CP084492:4100604-4100978 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A486RAG1