Detailed information of TA system TA178955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-ChpS
• Location: 213441..214030
• Replicon: chromosome
• Accession: NZ_CP063849
• Organism: Paludibaculum fermentans strain P105

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: IRI77_RS00795
• Protein ID: WP_194450194.1
• Coordinates: 213698..214030 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: IRI77_RS00790
• Protein ID: WP_194450193.1
• Coordinates: 213441..213698 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRI77_RS00775	211154..211810	+	657	WP_194450191.1	hypothetical protein	-
IRI77_RS00780	212119..212859	-	741	WP_194450192.1	hypothetical protein	-
IRI77_RS00785	212785..213264	+	480	WP_194453528.1	OmpA family protein	-
IRI77_RS00790	213441..213698	+	258	WP_194450193.1	antitoxin	Antitoxin
IRI77_RS00795	213698..214030	+	333	WP_194450194.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IRI77_RS00800	214168..214779	-	612	WP_194450195.1	hypothetical protein	-
IRI77_RS00805	215878..216729	-	852	WP_194450196.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
IRI77_RS00810	216726..217328	-	603	WP_194450197.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	207366..230875	23509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (5-78)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11739.59 Da
• Isoelectric Point: 9.4892

>T178955 WP_194450194.1 NZ_CP063849:213698-214030 [Paludibaculum fermentans]
MKRCDIWLVGLDPSEGHEQKGRRRVLIVSPEAFNRVIKVPVVLPITSGGSFARTAGFAVSLSGAGTQTTGVVRCDQPRAL
DLGARGGKRLESVPDAIVEEVLARLAAIFE

Nucleotide

• Length: 333 bp

>T178955 NZ_CP084434:c572672-572502 [Staphylococcus lugdunensis]
ATGTCAAAAGAAAAAAAGAGTCGTTTAAATTATCATGAGGAAGAGAATGCGATGGTTGAAAATTTAGAAGACTTAAAAGA
GCTGGGCAAAGAAATGGAACACATCTCAGAGAAAAATGACGAAGAAAAAGTTAATCAATCGCATGATACATCAAAGTCTT
CAAATTCATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9145.71 Da
• Isoelectric Point: 9.7603

>AT178955 WP_194450193.1 NZ_CP063849:213441-213698 [Paludibaculum fermentans]
MYTTHLRKVGGSIMLAVPPALLDLLGLRPGVKVGLAVEGGQLVVKPHSRPRYTLDELLAQCDAKAVRSQEDREWARGKAA
GGELI

Nucleotide

• Length: 258 bp

>AT178955 NZ_CP084434:c573311-572712 [Staphylococcus lugdunensis]
ATGGCAATGAACTTTAAAGTATTTGAAAATAAAGATCAAGTTGCAGAGTATTCAGCAGATATCATTAGAAAACAATTTAA
CAATAATCCTACTACAATTGCTGGATTTCATTTAAACAAAGAGGCATCACCAGTACTTGATATGCTTAAAAAGAATGTTG
ACCAACATGCGGTTGACTTCAGCCAAATTAACATTCTTGACTATGATCAAAATCAAGAATATTTCAAAGCGCTGGGGGTT
CCTGAGAGTCAAATTTATCCTATCTCATACGATCAAGATGCTGAAGCGTTTATCTCTGATAAAATCAAAACAAAAGATAA
TAAAGGGAAACTAATATTACAAGTAGCTTCAATTGATGATCAAGGAAACCTTAATATCAGTCTACGTCAAGGATTGTTAA
ATGCTAGAGAAATTATCTTAGTCATTACAGGACATAATAAAAAAGAAGTAGTAGAAAAACTGTATAATGAAAATGGAAAA
TCTAATTTTGAACCTGCTGATTTAAAAGCACATCGTATGGTAACGGTTATTTTAGACGAAGCGGCAGCTGAAGGTTTGCC
AGAAGATGTACGTCATTACTTTACTGATCGGTTTGCTTAA