Detailed information of TA system TA178941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 37055..37535
• Replicon: plasmid pRW412b
• Accession: NZ_CP063841
• Organism: Sphingopyxis granuli strain RW412

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: ICN83_RS20425
• Protein ID: WP_212599244.1
• Coordinates: 37266..37535 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: ICN83_RS20420
• Protein ID: WP_212599243.1
• Coordinates: 37055..37282 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN83_RS20390 (ICN83_20435)	33381..33947	+	567	WP_212599237.1	DUF736 family protein	-
ICN83_RS20395 (ICN83_20440)	34061..35029	+	969	WP_212599238.1	SLOG family protein	-
ICN83_RS20400 (ICN83_20445)	35058..35402	+	345	WP_212599239.1	hypothetical protein	-
ICN83_RS20405 (ICN83_20450)	35460..35765	+	306	WP_212599240.1	hypothetical protein	-
ICN83_RS20410 (ICN83_20455)	36019..36465	+	447	WP_212599241.1	hypothetical protein	-
ICN83_RS20415 (ICN83_20460)	36636..36896	+	261	WP_212599242.1	hypothetical protein	-
ICN83_RS20420 (ICN83_20465)	37055..37282	+	228	WP_212599243.1	DUF6290 family protein	Antitoxin
ICN83_RS20425 (ICN83_20470)	37266..37535	+	270	WP_212599244.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICN83_RS20430 (ICN83_20475)	37519..37998	+	480	WP_212599245.1	hypothetical protein	-
ICN83_RS20435 (ICN83_20480)	38504..38863	+	360	WP_212599246.1	single-stranded DNA-binding protein	-
ICN83_RS20440 (ICN83_20485)	38916..39059	+	144	WP_156417120.1	hypothetical protein	-
ICN83_RS20445 (ICN83_20490)	39056..39736	+	681	WP_212599247.1	hypothetical protein	-
ICN83_RS20450 (ICN83_20495)	39947..40207	-	261	WP_212599248.1	hypothetical protein	-
ICN83_RS20455 (ICN83_20500)	41051..41329	+	279	WP_212599249.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..138422	138422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 9967.59 Da
• Isoelectric Point: 10.6070

>T178941 WP_212599244.1 NZ_CP063841:37266-37535 [Sphingopyxis granuli]
MAWTIELSGAAEKTLKKLDRQAAKRITSFLRERVADGEGPRSTGKALQGPLAGLWRYRVGDFRIICEIEDGKLVVLVVTI
GHRGDVYRR

Nucleotide

• Length: 270 bp

>T178941 NZ_CP084431:8-334 [Edwardsiella piscicida]
ATGATGCGCGGCGACCTTGTGACCATCGCCATGCAGGGCGACTTTGGCAAGCCACGGCCTGCGCTTGTGGTTCAGGCCAA
CCAGTTCAGCGAGCACGGCAGCGTTACCGTGCTGCCTGTCACCAGCACACTTAATGCCGCGCCGTTGCTGCGCGTCACGG
TTCAGCCGAGCGCAGAGAACGGTTTGCAGAAGCCATCGCAGGTGATGGTCGACAAGGCGATGACGGTGCTGCGCAGCAAG
GTAGGGCCAGCTTTCGGGCGCATCGACGCGGACGCACTGCTTGAGGTTGAGCGTTGCTTGGCCGTGTTCTTGGGGATTGC
GAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8627.63 Da
• Isoelectric Point: 4.3224

>AT178941 WP_212599243.1 NZ_CP063841:37055-37282 [Sphingopyxis granuli]
MATSVRLDAETEGRLDRLADLTGRTKAYYIREMIERSIEDMEDYYLAAKVVEDIRAGRETVSSLDDVERRLGLDN

Nucleotide

• Length: 228 bp

>AT178941 NZ_CP084431:13652-11 [Edwardsiella piscicida]