Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81846..82489 Replicon plasmid pCRKP66R-3
Accession NZ_CP063835
Organism Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag IRT47_RS28240 Protein ID WP_001044770.1
Coordinates 81846..82262 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag IRT47_RS28245 Protein ID WP_001261282.1
Coordinates 82259..82489 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRT47_RS28220 77949..78221 - 273 Protein_105 transposase -
IRT47_RS28225 78250..78947 - 698 Protein_106 IS1 family transposase -
IRT47_RS28230 79203..80225 - 1023 WP_000361404.1 helicase UvrD -
IRT47_RS28235 80210..81772 - 1563 WP_004206609.1 AAA family ATPase -
IRT47_RS28240 81846..82262 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
IRT47_RS28245 82259..82489 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IRT47_RS28250 82446..82907 + 462 WP_014343465.1 hypothetical protein -
IRT47_RS28255 83068..84012 + 945 WP_011977810.1 hypothetical protein -
IRT47_RS28260 84049..84441 + 393 WP_011977811.1 hypothetical protein -
IRT47_RS28265 84499..85020 + 522 WP_013214008.1 hypothetical protein -
IRT47_RS28270 85066..85269 + 204 WP_011977813.1 hypothetical protein -
IRT47_RS28275 85299..86303 + 1005 WP_011977814.1 hypothetical protein -
IRT47_RS28280 86487..87266 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 - 1..128675 128675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T178932 WP_001044770.1 NZ_CP063835:c82262-81846 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T178932 NZ_CP084429:c1130508-1130305 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT178932 WP_001261282.1 NZ_CP063835:c82489-82259 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT178932 NZ_CP084429:c1130897-1130529 [Edwardsiella piscicida]
ATGGATGAGTACACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAATCTACAGCTTAATGAGCTGATTGAGC
ATATTGCCAGCGTCGCGCAATCATTTAAGATCAAATATCCGCATCATTCGGATTTAGCCGAAATATTGGATGATTATCTG
GATGAAACCTATGCCCTGTTTGGCGCCTATAGCGTCAGTGAAACGGCGCTCCGGCACTGGCTGCGCACCAAGCGGCGGGT
GGCATACTGCCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References