Detailed information of TA system TA178927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 142748..143418
• Replicon: plasmid pCRKP66R-2
• Accession: NZ_CP063834
• Organism: Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IRT47_RS27295
• Protein ID: WP_004213072.1
• Coordinates: 142748..143191 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IRT47_RS27300
• Protein ID: WP_004213073.1
• Coordinates: 143188..143418 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRT47_RS27260	138159..138434	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IRT47_RS27265	138497..138988	+	492	WP_011251327.1	DM13 domain-containing protein	-
IRT47_RS27270	139037..139957	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IRT47_RS27275	140048..140454	+	407	Protein_148	GAF domain-containing protein	-
IRT47_RS27280	140969..141604	-	636	Protein_149	response regulator	-
IRT47_RS27285	142021..142314	+	294	Protein_150	transposase	-
IRT47_RS27290	142318..142734	+	417	Protein_151	restriction endonuclease	-
IRT47_RS27295	142748..143191	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRT47_RS27300	143188..143418	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IRT47_RS27305	144026..145159	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IRT47_RS27310	145175..145468	+	294	WP_004213076.1	hypothetical protein	-
IRT47_RS27315	145458..145664	-	207	WP_004213077.1	hypothetical protein	-
IRT47_RS27320	146016..146306	+	291	WP_004213078.1	hypothetical protein	-
IRT47_RS27325	146296..147195	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..215999	215999

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T178927 WP_004213072.1 NZ_CP063834:c143191-142748 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T178927 NZ_CP084428:881715-882083 [Edwardsiella piscicida]
ATGAAAAATCTACCTGCTACAATTTCGCTGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAATCTGGCAAATGTTACTTAC
TCGTCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGGGAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT178927 WP_004213073.1 NZ_CP063834:c143418-143188 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT178927 NZ_CP084428:881377-881718 [Edwardsiella piscicida]
ATGAGCAACCCCACATGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCAACTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCCCTCAGCGCAAACA
AATTTCACGAGAGCAAACATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620