Detailed information of TA system TA178926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 103843..104753
• Replicon: plasmid pCRKP66R-2
• Accession: NZ_CP063834
• Organism: Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: IRT47_RS27060
• Protein ID: WP_004026354.1
• Coordinates: 103843..104313 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: IRT47_RS27065
• Protein ID: WP_004026357.1
• Coordinates: 104310..104753 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRT47_RS27040	99255..99653	-	399	WP_004212796.1	H-NS histone family protein	-
IRT47_RS27045	99990..100484	-	495	WP_004212794.1	thermonuclease family protein	-
IRT47_RS27050	100662..101611	+	950	Protein_103	DUF1173 family protein	-
IRT47_RS27055	102997..103694	-	698	Protein_104	IS1 family transposase	-
IRT47_RS27060	103843..104313	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
IRT47_RS27065	104310..104753	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
IRT47_RS27070	104854..105357	-	504	Protein_107	DUF4113 domain-containing protein	-
IRT47_RS27075	105563..106543	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
IRT47_RS27080	106609..107916	+	1308	Protein_109	FepA family TonB-dependent siderophore receptor	-
IRT47_RS27085	107978..108199	+	222	WP_004213615.1	hypothetical protein	-
IRT47_RS27090	108342..109244	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..215999	215999
	-	inside	IScluster/Tn	-	-	97804..106543	8739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T178926 WP_004026354.1 NZ_CP063834:c104313-103843 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T178926 NZ_CP084427:c3120180-3119764 [Edwardsiella piscicida]
GTGGTCCTTTGGCGCTGTAATGTTCACGTCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCACGGCACGCTGGTACT
GGCGATCCTGCTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTGGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGCCGGATTGCGCGTAACCGCGGTGAGCTGCAGCTGCTGGCGCCGCAGGTGTGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGACGTCCTTGGGTTAGCGATCTGGGGGCGCTGCTGATCCTGCAGAGTACCCATGGTGCGCCGTG
CCGCCGCCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCACGCTGCTCGATGCGG
GCGACGATCGCGCATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT178926 WP_004026357.1 NZ_CP063834:c104753-104310 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT178926 NZ_CP084427:c3120427-3120161 [Edwardsiella piscicida]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGCGAGCTGGATATCGCCATCATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGCCTGCTGGCCTGTGACGATCCTGAGC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGATGACGCCGATCTGGCCCGCATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGTCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9