Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5312257..5312882 Replicon chromosome
Accession NZ_CP063833
Organism Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IRT47_RS26170 Protein ID WP_002882817.1
Coordinates 5312257..5312640 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IRT47_RS26175 Protein ID WP_004150355.1
Coordinates 5312640..5312882 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRT47_RS26155 5309623..5310525 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IRT47_RS26160 5310522..5311157 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IRT47_RS26165 5311154..5312083 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IRT47_RS26170 5312257..5312640 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IRT47_RS26175 5312640..5312882 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IRT47_RS26180 5313087..5314004 + 918 WP_002882812.1 alpha/beta hydrolase -
IRT47_RS26185 5314018..5314959 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IRT47_RS26190 5315004..5315441 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IRT47_RS26195 5315438..5316298 - 861 WP_002882807.1 virulence factor BrkB family protein -
IRT47_RS26200 5316292..5316891 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T178924 WP_002882817.1 NZ_CP063833:c5312640-5312257 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T178924 NZ_CP084427:c1082847-1082644 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT178924 WP_004150355.1 NZ_CP063833:c5312882-5312640 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT178924 NZ_CP084427:c1083236-1082868 [Edwardsiella piscicida]
ATGGATGAGTACACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAATCTACAGCTTAATGAGTTGATTGAGC
ATATTGCCAGCGTCGCGCAATCATTTAAGATCAAATATCCGCATCATTCGGATTTAGCCGAAATATTGGATGATTATCTG
GATGAAACCTATGCCCTGTTTGGCGCCTATAGCGTCAGTGAAACGGCGCTCCGGCACTGGCTGCGCACCAAGCGGCGGGT
GGCATACTGCCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References