Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4727949..4728759 Replicon chromosome
Accession NZ_CP063833
Organism Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IRT47_RS23385 Protein ID WP_002887280.1
Coordinates 4727949..4728482 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IRT47_RS23390 Protein ID WP_002887278.1
Coordinates 4728493..4728759 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRT47_RS23380 4726780..4727901 + 1122 WP_002887282.1 cupin domain-containing protein -
IRT47_RS23385 4727949..4728482 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
IRT47_RS23390 4728493..4728759 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IRT47_RS23395 4728862..4730295 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
IRT47_RS23400 4730285..4730968 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IRT47_RS23405 4731140..4732525 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
IRT47_RS23410 4732543..4732887 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T178921 WP_002887280.1 NZ_CP063833:c4728482-4727949 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T178921 NZ_CP084424:c1083000-1082797 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT178921 WP_002887278.1 NZ_CP063833:c4728759-4728493 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT178921 NZ_CP084424:c1083389-1083021 [Edwardsiella piscicida]
ATGGATGAGTACACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAATCTACAGCTTAATGAGTTGATTGAGC
ATATTGCCAGCGTCGCGCAATCATTTAAGATCAAATATCCGCATCATTCGGATTTAGCCGAAATATTGGATGATTATCTG
GATGAAACCTATGCCCTGTTTGGCGCCTATAGCGTCAGTGAAACGGCGCTCCGGCACTGGCTGCGCACCAAGCGGCGGGT
GGCATACTGCCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References