Detailed information of TA system TA178904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3697753..3698393
• Replicon: chromosome
• Accession: NZ_CP063832
• Organism: Pseudomonas entomophila strain 23S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IM733_RS16355
• Protein ID: WP_248917603.1
• Coordinates: 3697983..3698393 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: IM733_RS16350
• Protein ID: WP_011535889.1
• Coordinates: 3697753..3697983 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM733_RS16330	3693154..3694464	-	1311	WP_283107483.1	precorrin-3B synthase	-
IM733_RS16335	3694647..3695858	+	1212	WP_248917600.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
IM733_RS16340	3695851..3696945	+	1095	WP_248917601.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
IM733_RS16345	3696942..3697670	+	729	WP_248917602.1	cobalt-precorrin-6A reductase	-
IM733_RS16350	3697753..3697983	+	231	WP_011535889.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IM733_RS16355	3697983..3698393	+	411	WP_248917603.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IM733_RS16360	3698465..3698902	+	438	WP_248917604.1	NfeD family protein	-
IM733_RS16365	3698930..3699784	+	855	WP_248917605.1	SPFH domain-containing protein	-
IM733_RS16370	3699897..3700286	+	390	WP_248917606.1	DUF2946 domain-containing protein	-
IM733_RS16375	3700334..3700816	+	483	WP_248917607.1	copper chaperone PCu(A)C	-
IM733_RS16380	3700842..3701273	+	432	WP_248917608.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15170.50 Da
• Isoelectric Point: 9.5443

>T178904 WP_248917603.1 NZ_CP063832:3697983-3698393 [Pseudomonas entomophila]
MLRYMLDTNICIFTIKNKPQAVREAFNRHHGQLAISTVTLMELIYGAEKSATPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTPAR

Nucleotide

• Length: 411 bp

>T178904 NZ_CP084413:c114400-114119 [Pseudomonas sp. HS-18]
ATGAGCCTGCAGTGGACGCACAAGGCCGCAGCCGACCTGGACGCCATCTATGACCACTACGTCGTGCTGATCGGCCCGGA
GAAGGCATTAAGGGCGATCCAGGACATCGTCGAGCAGGTCAGCCCGCTGGCGAACCTGGACCTGAGCAGCAGCGGCGTGC
CGAGCGAAGTCCCCGGCGTGCGCGAACTGAGTGTCGAGCGCTGGCCGTACCAGGCCGCTTTCCGGGTAAAGGGCCGTTCT
GTACAGATTTTGCGCATCGACCGTGTGGATAACCCGGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT178904 WP_011535889.1 NZ_CP063832:3697753-3697983 [Pseudomonas entomophila]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT178904 NZ_CP084413:c114624-114397 [Pseudomonas sp. HS-18]
GTGACCACCGTTGTTTCGTTCCGCGCCGATGACAGCCTCGTGGCGGCGCTGGACCAACTGGCCCGCGCCACCCACCGGGA
CCGGCCGTATCACCTGCGCCAGGCCCTGGCGCAGTACCTGGAGCGCCAGAGCTGGCACGTGGCGGCCATCGATGAAGGCG
TGGCGGACGCCGATGCCGGCCACCTGCTGGAGCACGCCGCCATCGAAGGCAAGTGGGGGCTGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6