Detailed information of TA system TA178892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3129392..3130079
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: RJtmp_RS14975
• Protein ID: WP_003414624.1
• Coordinates: 3129636..3130079 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: RJtmp_RS14970
• Protein ID: WP_003414620.1
• Coordinates: 3129392..3129649 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS14950 (RJtmp_002958)	3124712..3125566	-	855	WP_003414604.1	GNAT family N-acetyltransferase	-
RJtmp_RS14955 (RJtmp_002959)	3125622..3126785	-	1164	WP_003414608.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
RJtmp_RS14960 (RJtmp_002960)	3126802..3128016	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
RJtmp_RS14965 (RJtmp_002961)	3128024..3129265	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
RJtmp_RS14970 (RJtmp_002962)	3129392..3129649	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
RJtmp_RS14975 (RJtmp_002963)	3129636..3130079	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJtmp_RS14980 (RJtmp_002964)	3130159..3130821	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
RJtmp_RS14985 (RJtmp_002965)	3130917..3131108	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
RJtmp_RS14990 (RJtmp_002966)	3131440..3133188	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
RJtmp_RS14995 (RJtmp_002967)	3133284..3133865	+	582	WP_003414644.1	fasciclin domain-containing protein	-
RJtmp_RS15000 (RJtmp_002968)	3133965..3134231	+	267	WP_031708549.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T178892 WP_003414624.1 NZ_CP063804:3129636-3130079 [Mycobacterium orygis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T178892 NZ_CP084405:1423421-1423843 [Sphingobium sp. CFD-2]
ATGATCGTTCTGGACACCAATGTCGTTTCCGAGGCGATGAAGCCGGAACCGGCGCCCGCCGTGCGCGACTGGCTGGATGA
GCAGGCCGCCGAAACCCTCTATCTCTCCAGCGTCACCGTCGCGGAACTCCTGTTCGGCATCGGCGCGCTGCCGGACGGGC
AGCGCAAGCGGAAGCTCGCCGCAACCCTCGACGGGCTTCTCGGCCTGTTCGACGGCAGAATCCTTCCCTTCGATACGGAT
GCCGCTCGCCGCTATGCCGACCTCGCCGTTGCGGCGCGCAACGCGGGCAAGGGTTTTCCGACGCCGGACGGTTACATCGC
GGCCATCGCGACTGCCCATGGCTTTGCAGTCGCCACGCGCGATGCCAGCGCCTTCAAGGCGGCGGGTGTCCCGATCATCG
ACCCTTGGACGGCCGGGCACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT178892 WP_003414620.1 NZ_CP063804:3129392-3129649 [Mycobacterium orygis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT178892 NZ_CP084405:1423170-1423424 [Sphingobium sp. CFD-2]
ATGCCCGCCGTCACCATTCGCAACCTGTCAGACGAGGCGCACCGCGCCCTCAAGGTGCGCGCGGCCCATCATGGTCGCAG
CACCGAGGCCGAAATCCGCGATATTCTGGAAGAGGCGGTGCGTCCCGCCAACCGCCTGCGCATCGGTTCCGCCATGGCGG
CGTTGAGCCGGGAGGCAGGCTTGACCAATGCCGATTTCGAGGCGCTGGAACAGAGCCGCGACCGGACGCCCGCCGTGCCT
ATGAGCTTTGAATGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0