Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3082875..3083479 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag RJtmp_RS14750 Protein ID WP_003414492.1
Coordinates 3082875..3083267 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag RJtmp_RS14755 Protein ID WP_003414495.1
Coordinates 3083264..3083479 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS14720 (RJtmp_002911) 3078025..3078813 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
RJtmp_RS14725 (RJtmp_002912) 3079147..3079692 - 546 WP_003904931.1 DUF1802 family protein -
RJtmp_RS14730 (RJtmp_002913) 3079964..3080848 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
RJtmp_RS14735 (RJtmp_002914) 3080851..3081738 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
RJtmp_RS14740 (RJtmp_002915) 3082043..3082588 - 546 WP_003899500.1 DUF1802 family protein -
RJtmp_RS14745 (RJtmp_002916) 3082585..3082854 - 270 WP_003414489.1 DUF2277 family protein -
RJtmp_RS14750 (RJtmp_002917) 3082875..3083267 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
RJtmp_RS14755 (RJtmp_002918) 3083264..3083479 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RJtmp_RS14760 (RJtmp_002919) 3083526..3084275 + 750 WP_003414497.1 enoyl-CoA hydratase -
RJtmp_RS14765 (RJtmp_002920) 3084354..3085436 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
RJtmp_RS14770 (RJtmp_002921) 3085429..3086739 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
RJtmp_RS14775 (RJtmp_002922) 3086742..3087569 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
RJtmp_RS14780 (RJtmp_002923) 3087566..3088477 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T178890 WP_003414492.1 NZ_CP063804:c3083267-3082875 [Mycobacterium orygis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T178890 NZ_CP084399:72690-72971 [Sphingobium sp. CFD-1]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT178890 WP_003414495.1 NZ_CP063804:c3083479-3083264 [Mycobacterium orygis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT178890 NZ_CP084399:72440-72703 [Sphingobium sp. CFD-1]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References