Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3057943..3058513 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag RJtmp_RS14600 Protein ID WP_003414166.1
Coordinates 3057943..3058299 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag RJtmp_RS14605 Protein ID WP_003901465.1
Coordinates 3058283..3058513 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS14580 (RJtmp_002884) 3053395..3055083 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
RJtmp_RS14585 (RJtmp_002885) 3055087..3055413 - 327 WP_003414157.1 hypothetical protein -
RJtmp_RS14590 (RJtmp_002886) 3055586..3056173 + 588 WP_003914429.1 DUF3558 family protein -
RJtmp_RS14595 (RJtmp_002887) 3056192..3057841 + 1650 WP_003414164.1 CocE/NonD family hydrolase -
RJtmp_RS14600 (RJtmp_002888) 3057943..3058299 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
RJtmp_RS14605 (RJtmp_002889) 3058283..3058513 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
RJtmp_RS14610 (RJtmp_002890) 3058556..3059599 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
RJtmp_RS14615 (RJtmp_04375) 3059598..3060065 + 468 WP_003414177.1 DUF1778 domain-containing protein -
RJtmp_RS14620 (RJtmp_002892) 3060241..3060495 - 255 WP_003917684.1 hypothetical protein -
RJtmp_RS14625 (RJtmp_002893) 3060643..3061047 + 405 WP_003414181.1 hypothetical protein -
RJtmp_RS14630 (RJtmp_002894) 3061044..3061235 + 192 WP_003414184.1 hypothetical protein -
RJtmp_RS14635 (RJtmp_04380) 3061452..3061712 + 261 Protein_2890 transposase -
RJtmp_RS14640 (RJtmp_002896) 3062822..3063079 + 258 WP_003899489.1 hypothetical protein -
RJtmp_RS14645 (RJtmp_002897) 3063184..3063495 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T178888 WP_003414166.1 NZ_CP063804:c3058299-3057943 [Mycobacterium orygis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T178888 NZ_CP084398:2860145-2860516 [Sphingobium sp. CFD-1]
ATGATCTTGGTCGACACTTCGGTCTGGATTGATCATTTGCGTCACGATGATCCCGTCCTTTCCCAATCGCTCAGCAAACG
GCAGGTTCTCTCCCATCCTTTCATCATCGGGGAGTTGGCACTGGGCAGCCTTCGGCAAAGAGACCTCATCCTCGAAGCGC
TACGTGGATTGCCGTCAGTTATTGTCGCCCACGACGAGGAAGTTCATGCCTTTATAGACCTTCATAGGCTCTTCGGTATT
GGCATCGGCTACATCGATGCACACCTGCTGGCAGCAACCCTTCTAACGCCTGACGTGCAATTCTGGACACGTGACAAGCG
GTTGCGGGCCGCGGCCTTGCGTCTGGGCGTGGATGCGAATCTGGATCACTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT178888 WP_003901465.1 NZ_CP063804:c3058513-3058283 [Mycobacterium orygis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT178888 NZ_CP084398:2859951-2860148 [Sphingobium sp. CFD-1]
ATGCGGACTACAGTCGCTCTCGATGATGAACTTGTCGCGAAGGCACAGGCTTTCACCGGCCTGACCGAAAAATCGTCACT
CGTTCGCGAAGCGCTGAAAGCCCTCATAGAAAGGGAGAGCTCAGCGCGGCTTGTCCGCCTTGGTGGCACTGAGCCTCATG
GTGAAGCCGCGCCACGCCGACGCCCCGATCCTGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References