Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2827078..2827715 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag RJtmp_RS13340 Protein ID WP_003413180.1
Coordinates 2827078..2827473 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag RJtmp_RS13345 Protein ID WP_003413183.1
Coordinates 2827470..2827715 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS13300 (RJtmp_002631) 2822482..2823693 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
RJtmp_RS13305 (RJtmp_002632) 2823820..2824479 + 660 WP_229306040.1 LppA family lipoprotein -
RJtmp_RS13310 (RJtmp_002633) 2824476..2825137 + 662 Protein_2630 LppA family lipoprotein -
RJtmp_RS13315 (RJtmp_002634) 2825134..2825412 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
RJtmp_RS13320 (RJtmp_002635) 2825505..2825918 + 414 WP_003413164.1 PIN domain nuclease -
RJtmp_RS13325 (RJtmp_002636) 2825957..2826214 + 258 WP_003413167.1 CopG family transcriptional regulator -
RJtmp_RS13330 (RJtmp_002637) 2826211..2826588 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
RJtmp_RS13335 (RJtmp_002638) 2826604..2826978 - 375 WP_003413177.1 hypothetical protein -
RJtmp_RS13340 (RJtmp_002639) 2827078..2827473 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
RJtmp_RS13345 (RJtmp_002640) 2827470..2827715 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
RJtmp_RS13350 (RJtmp_002641) 2828126..2828545 - 420 WP_003413190.1 A24 family peptidase -
RJtmp_RS13355 (RJtmp_002642) 2828557..2829366 - 810 WP_003413193.1 shikimate dehydrogenase -
RJtmp_RS13360 (RJtmp_002643) 2829363..2830616 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
RJtmp_RS13365 (RJtmp_002644) 2830609..2831121 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T178884 WP_003413180.1 NZ_CP063804:c2827473-2827078 [Mycobacterium orygis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T178884 NZ_CP084398:c899907-899617 [Sphingobium sp. CFD-1]
ATGAAGACGATCAAGACCACCGCCGTTTATGACAACTGGATCGAAGGCCTCAGGGATCGTCAGGCAGTCGCTCGCATTCG
TATTCGCATCGACCGTCTTGCCATGGGCAACCGGGGCGACCACCGCAATCTTAAGGCGGGCGTGGTGGAACTGAAAATCG
ACTTCGGCCCCGGCTATCGGGTCTACTGCACGGAGCGCGCTGGCGAGATCGTCATCCTTCTCTGCGGCGGCACGAAGAAG
GGGCAGGACGCGGACATCGCTCTGGCAGAGAAAATGGCGAAGGAACTGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT178884 WP_003413183.1 NZ_CP063804:c2827715-2827470 [Mycobacterium orygis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT178884 NZ_CP084398:c899617-899336 [Sphingobium sp. CFD-1]
ATGGCAATCGAACTGAAAAACTATGACGTGGCGGAACACCTCAGCAATGATGAGGAAATTCGCCTCTATCTTGAAGCCGC
CTTTGAGGATGGCGATCCGGCGATTATCAAGAACGCTATCTCCAATGTCGTCCGCGCTCGCGGCATGACGGCGATGGCGA
AGGAATCGGGCGTCTCCCGTGAAACGCTTTACAAGGCCTTCGCGGAGAACGGGAACCCGACACTGGACACGCTGATGAAG
GTGACGAAAGCGCTGGGCGTGAGATTGGCGATAGCGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References