Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2765432..2766084 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag RJtmp_RS13040 Protein ID WP_003412752.1
Coordinates 2765659..2766084 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag RJtmp_RS13035 Protein ID WP_003412749.1
Coordinates 2765432..2765653 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS13020 (RJtmp_002575) 2763672..2763878 - 207 WP_003899347.1 hypothetical protein -
RJtmp_RS13025 (RJtmp_002576) 2764014..2764637 + 624 WP_003412743.1 TIGR00725 family protein -
RJtmp_RS13030 (RJtmp_002577) 2764627..2765379 + 753 WP_003412746.1 hypothetical protein -
RJtmp_RS13035 (RJtmp_002578) 2765432..2765653 + 222 WP_003412749.1 antitoxin Antitoxin
RJtmp_RS13040 (RJtmp_002579) 2765659..2766084 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
RJtmp_RS13045 (RJtmp_002580) 2766107..2767288 - 1182 WP_003412753.1 dihydrolipoamide acetyltransferase family protein -
RJtmp_RS13050 (RJtmp_002581) 2767285..2768331 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
RJtmp_RS13055 (RJtmp_002582) 2768342..2769445 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
RJtmp_RS13060 (RJtmp_002583) 2769704..2770525 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
RJtmp_RS13065 (RJtmp_002584) 2770522..2771079 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T178880 WP_003412752.1 NZ_CP063804:2765659-2766084 [Mycobacterium orygis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T178880 NZ_CP063804:2765659-2766084 [Mycobacterium orygis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT178880 WP_003412749.1 NZ_CP063804:2765432-2765653 [Mycobacterium orygis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT178880 NZ_CP063804:2765432-2765653 [Mycobacterium orygis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References