Detailed information of TA system TA178875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2232444..2233070
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: RJtmp_RS10525
• Protein ID: WP_003410075.1
• Coordinates: 2232672..2233070 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RJtmp_RS10520
• Protein ID: WP_019283586.1
• Coordinates: 2232444..2232671 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS10510 (RJtmp_002075)	2230483..2230827	-	345	WP_003410065.1	ferredoxin family protein	-
RJtmp_RS10515 (RJtmp_002076)	2231016..2232185	-	1170	WP_003899126.1	ATP-binding protein	-
RJtmp_RS10520 (RJtmp_002077)	2232444..2232671	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RJtmp_RS10525 (RJtmp_002078)	2232672..2233070	+	399	WP_003410075.1	PIN domain nuclease	Toxin
RJtmp_RS10530 (RJtmp_002079)	2233253..2233684	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
RJtmp_RS10535 (RJtmp_002080)	2233785..2234219	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
RJtmp_RS10540 (RJtmp_002081)	2234656..2234835	-	180	Protein_2084	hypothetical protein	-
RJtmp_RS10545 (RJtmp_002082)	2234923..2235543	+	621	WP_003410086.1	IS110 family transposase	-
RJtmp_RS10550 (RJtmp_002083)	2235497..2236087	+	591	WP_003410089.1	transposase	-
RJtmp_RS10555 (RJtmp_002084)	2236215..2237471	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T178875 WP_003410075.1 NZ_CP063804:2232672-2233070 [Mycobacterium orygis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T178875 NZ_CP084397:1469128-1469610 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT178875 WP_019283586.1 NZ_CP063804:2232444-2232671 [Mycobacterium orygis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT178875 NZ_CP084397:1468874-1469140 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 4CHG