Detailed information of TA system TA178866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1776165..1776778
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: RJtmp_RS08365
• Protein ID: WP_003407786.1
• Coordinates: 1776374..1776778 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: RJtmp_RS08360
• Protein ID: WP_009935474.1
• Coordinates: 1776165..1776368 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS08345 (RJtmp_001645)	1771429..1774332	+	2904	WP_003407779.1	MMPL family transporter	-
RJtmp_RS08350 (RJtmp_001646)	1774342..1774788	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
RJtmp_RS08355 (RJtmp_001647)	1774823..1776112	+	1290	WP_003407781.1	threonine ammonia-lyase	-
RJtmp_RS08360 (RJtmp_001648)	1776165..1776368	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RJtmp_RS08365 (RJtmp_001649)	1776374..1776778	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
RJtmp_RS08370 (RJtmp_001650)	1776795..1778537	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
RJtmp_RS08375 (RJtmp_001651)	1778530..1780827	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T178866 WP_003407786.1 NZ_CP063804:1776374-1776778 [Mycobacterium orygis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T178866 NZ_CP084390:c64106-63855 [Lactobacillus helsingborgensis]
ATGAATAAATATAAAAAATTATTGGAAAGATTTAATAGCAAACCAAATGATTTCACTTTTGAAGAAGCCAAAAAGTTATT
ATCATATTCTGATTTTAAACTGGTTATGAAGGGACATTCAAGTGGTTCACGTGTACAGTTTAGATGCGATAATGTTAAAA
TTGATTTACATAGACCACATCCTAAGAACTATTTAAAGAAATATCAGCTTGAGGATATTGAAGAGAGCTTAAAGAAAGCA
GGTAAATTATGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT178866 WP_009935474.1 NZ_CP063804:1776165-1776368 [Mycobacterium orygis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT178866 NZ_CP084390:c63858-63595 [Lactobacillus helsingborgensis]
ATGAGTTTATTATCGTACAAAGGTTATACAGGCTCGATTGAGTTTTCTGCTGAGGACAATATCATGTATGGTAGAGTTTT
AGGCCTTAAAAACTCAATAATTTCATATGAAGGAAAATCATTTCAAGAACTTGAAAATGACTTTAAAGATGGAATTAATG
ATTATCTGGCTAGCTGTATTGCGGAACACGAACAACCAGAAACAAGTAGCAAAGATAGCGTAAGAAAAGATTGGAATGTA
TTGTTAGATCAAGAACTTGCCTAG

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46