Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1695511..1696127 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag RJtmp_RS08015 Protein ID WP_003407593.1
Coordinates 1695810..1696127 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829CBK3
Locus tag RJtmp_RS08010 Protein ID WP_003407590.1
Coordinates 1695511..1695813 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS08000 (RJtmp_001576) 1691397..1693244 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
RJtmp_RS08005 (RJtmp_001577) 1693245..1695497 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
RJtmp_RS08010 (RJtmp_001578) 1695511..1695813 + 303 WP_003407590.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
RJtmp_RS08015 (RJtmp_001579) 1695810..1696127 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
RJtmp_RS08020 (RJtmp_001580) 1696124..1697128 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
RJtmp_RS08025 (RJtmp_001581) 1697181..1698470 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
RJtmp_RS08030 (RJtmp_001582) 1698543..1699268 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
RJtmp_RS08035 (RJtmp_001583) 1699374..1699565 - 192 WP_003407609.1 dodecin family protein -
RJtmp_RS08040 (RJtmp_04255) 1699647..1700045 + 399 WP_003900351.1 hypothetical protein -
RJtmp_RS08045 (RJtmp_001585) 1700090..1701118 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T178865 WP_003407593.1 NZ_CP063804:1695810-1696127 [Mycobacterium orygis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T178865 NZ_CP084389:1701249-1701581 [Lactobacillus helsingborgensis]
ATGGTAATTAAGCAAGGATCTATTCTATTAATGGATTTAAGTCCTAAACAGGGGCATGAACAATCTGGCAAACGACCTTA
TTTATATTTAAGCTATAAAGGGGTTGAAAAATACGCTCATATTGCTGTGTTTGCTCCTATATCTACTACTAAAAGACGCT
ACCCATTATATCAAGAATTACCTGCTGTATCTCAAACAAAAGGCGTAGTCATGCTAGACCAGCTAGTTACAATCGATTAT
CATAATCAATCATTTCAATATTTAGAGCAACTACCTGATGACTATATGGAAAAGATACTCAAAATTGTAAAAGTAGTTTT
TCAGAAAGATTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11120.38 Da        Isoelectric Point: 8.0518

>AT178865 WP_003407590.1 NZ_CP063804:1695511-1695813 [Mycobacterium orygis]
VPFLVALSGIISGVCDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT178865 NZ_CP084389:1701004-1701255 [Lactobacillus helsingborgensis]
ATGATTCGTGCTACTATATCTAAATGGGGTAATTCTAAAGGTATCCGATTAAACCAAGCAGTCTTGCAAGCATTAAACGC
TACTATAGGAGATCAGTTAAACTTTAAAATTGAGGACAACAAAGTAATAATGTCAAAGCAAGATGATTCAACTGATTTAA
CTTTTGAAGAACTTTTTAAAGATTATTCAGGTGAAGAATTTGATACCGAAATTAAGTTACCAGATGAGAGAAAAGGAAAT
GAAAGATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBK3

References