Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1583029..1583684 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag RJtmp_RS07515 Protein ID WP_003407268.1
Coordinates 1583029..1583430 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag RJtmp_RS07520 Protein ID WP_003407272.1
Coordinates 1583427..1583684 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS07495 (RJtmp_001473) 1578136..1579614 - 1479 WP_003407257.1 NAD(P)/FAD-dependent oxidoreductase -
RJtmp_RS07500 (RJtmp_001474) 1579611..1580996 - 1386 WP_003407260.1 cytochrome P450 -
RJtmp_RS07505 (RJtmp_001475) 1581074..1582108 + 1035 WP_003407264.1 AraC family transcriptional regulator -
RJtmp_RS07510 (RJtmp_001476) 1582268..1582774 - 507 Protein_1479 PE family protein -
RJtmp_RS07515 (RJtmp_001477) 1583029..1583430 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
RJtmp_RS07520 (RJtmp_001478) 1583427..1583684 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
RJtmp_RS07525 (RJtmp_001479) 1583767..1584726 - 960 WP_003407276.1 alpha/beta hydrolase -
RJtmp_RS07530 (RJtmp_001480) 1584751..1585713 - 963 WP_003407279.1 alpha/beta hydrolase -
RJtmp_RS07535 (RJtmp_001481) 1585712..1586449 + 738 WP_031647000.1 lysoplasmalogenase -
RJtmp_RS07540 (RJtmp_001482) 1586530..1588497 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T178864 WP_003407268.1 NZ_CP063804:c1583430-1583029 [Mycobacterium orygis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T178864 NZ_CP084385:116774-117025 [Lactobacillus helsingborgensis]
ATGAATAAATATAAAAAATTATTGGAAAGATTTAATAGCAAACCAAATGATTTCACTTTTGAAGAAGCCAAAAAGTTATT
ATCATATTCTGATTTTAAACTGGTTATGAAGGGACATTCAAGTGGTTCACGTGTACAGTTTAGATGCGATAATGTTAAAA
TTGATTTACATAGACCACATCCTAAGAACTATTTAAAGAAATATCAGCTTGAGGATATTGAAGAGGGCTTAAAGAAAGCA
GGTAAATTATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT178864 WP_003407272.1 NZ_CP063804:c1583684-1583427 [Mycobacterium orygis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT178864 NZ_CP084385:117022-117285 [Lactobacillus helsingborgensis]
ATGAGTTTATTATCGTACAAAGGTTATACAGGCTCGATTGAGTTTTCTGCTGAGGACAATATCATGTATGGTAGAGTTTT
AGGCCTTAAAAACTCAATAATTTCATATGAAGGAAAATCATTTCAAGAACTTGAAAATGACTTTAAAGATGGAATTAATG
ATTATCTGGCTAGCTGTATTGCGGAACACGAACAACCAGAAACAAGTAGCAAAGATAGCGTAAGAAAAGATTGGAATGTA
TTGTTAGATCAAGAACTTGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References