Detailed information of TA system TA178861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1391503..1392191
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: RJtmp_RS06665
• Protein ID: WP_003406304.1
• Coordinates: 1391760..1392191 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: RJtmp_RS06660
• Protein ID: WP_003406302.1
• Coordinates: 1391503..1391763 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS06640 (RJtmp_001303)	1387080..1387904	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
RJtmp_RS06645 (RJtmp_001304)	1387909..1389090	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
RJtmp_RS06650 (RJtmp_001305)	1389167..1390267	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
RJtmp_RS06655 (RJtmp_001306)	1390438..1391427	+	990	WP_003406301.1	malate dehydrogenase	-
RJtmp_RS06660 (RJtmp_001307)	1391503..1391763	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
RJtmp_RS06665 (RJtmp_001308)	1391760..1392191	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJtmp_RS06670 (RJtmp_001309)	1392214..1393894	-	1681	Protein_1317	PE family protein	-
RJtmp_RS06675 (RJtmp_001310)	1394074..1394934	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
RJtmp_RS06680 (RJtmp_001311)	1395015..1395845	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
RJtmp_RS06685 (RJtmp_001312)	1395926..1396195	-	270	WP_003406309.1	type II toxin-antitoxin system mRNA interferase RelE	-
RJtmp_RS06690 (RJtmp_001313)	1396192..1396461	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T178861 WP_003406304.1 NZ_CP063804:1391760-1392191 [Mycobacterium orygis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T178861 NZ_CP084357:1609500-1609847 [Streptomyces sp. WA6-1-16]
ATGCGTAGGGGCGACATCCACCTCGTGGACCTGGAGCCCGCACGGGGGAGCGAAGCCAACAAGGTGCGTCCGGCGGTGAT
CGTCTCCAACAACGCCGCCAACCGGTCGGCCGAGGTGTCCGGCAGGGGCGTCGTCGCCGTGGTGCCCTTGACCTCGAACA
CTTCGCGGATCCTCAGCTTCCAGGTGCTTCTCACCGCGGAGGAGAGCCGCCTTCCGCAGAACTCCAAGGTTCAGTGCGAA
CAGATCCGGGCGGTCTCCCCCGACCGGATTCTGAAGCGCCTCGGTGAGGTGCCCCGTCAGCGCATGGCGGAGATCGACAA
GGCTCTGCGCCGCCACCTCGCCCTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT178861 WP_003406302.1 NZ_CP063804:1391503-1391763 [Mycobacterium orygis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT178861 NZ_CP084357:1609283-1609507 [Streptomyces sp. WA6-1-16]
ATGAAGATCAGTGTGAGCCTGCCTCAGGAGGATGTCGCCTTCGTCGACGAGTACGCGGCGAAGACCGACGCGGATTCCCG
GTCCGCTGTCATACACGCCGCTATCGAGCTGCTGCGCACCGCAGGCCTGGAGGCGGAGTACACCGAGGCGTTCCAGGAGT
GGGACGCGAGTGAGGACGCCGCTCTGTGGGACCGCACGGTGGCCGACGGGCTCACGGATGCGTAG

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6