Detailed information of TA system TA178860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244080..1244648
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: RJtmp_RS05975
• Protein ID: WP_003405865.1
• Coordinates: 1244274..1244648 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: RJtmp_RS05970
• Protein ID: WP_003405863.1
• Coordinates: 1244080..1244277 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS05950 (RJtmp_001170)	1240121..1240759	-	639	WP_003405849.1	lipid droplet-associated protein	-
RJtmp_RS05955 (RJtmp_001171)	1240849..1241856	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
RJtmp_RS05960 (RJtmp_001172)	1241873..1242856	-	984	WP_003405856.1	hypothetical protein	-
RJtmp_RS05965 (RJtmp_001173)	1242919..1243992	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
RJtmp_RS05970 (RJtmp_001174)	1244080..1244277	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RJtmp_RS05975 (RJtmp_001175)	1244274..1244648	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJtmp_RS05980 (RJtmp_001176)	1244851..1245549	+	699	WP_081608377.1	hypothetical protein	-
RJtmp_RS05985 (RJtmp_001177)	1245667..1245852	+	186	WP_003901093.1	hypothetical protein	-
RJtmp_RS05990 (RJtmp_001178)	1245779..1246054	-	276	WP_003405867.1	hypothetical protein	-
RJtmp_RS05995 (RJtmp_001179)	1246297..1246620	+	324	WP_003405871.1	putative quinol monooxygenase	-
RJtmp_RS06000 (RJtmp_001180)	1246635..1247333	-	699	WP_031646953.1	hypothetical protein	-
RJtmp_RS06005 (RJtmp_001181)	1247367..1247576	+	210	WP_003911400.1	hypothetical protein	-
RJtmp_RS06010 (RJtmp_001182)	1247528..1248298	-	771	Protein_1184	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T178860 WP_003405865.1 NZ_CP063804:1244274-1244648 [Mycobacterium orygis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T178860 NZ_CP084357:c913586-913335 [Streptomyces sp. WA6-1-16]
ATGCGCACGATGACCTACACGGAGTCCCGCGCGAATTACGCCGAGACCCTGAGCGCGGTCGTGGACGACCGTGAAGAGGT
CGTGGTCACGCGGGCCGGCCACGAGCCCGTGGTGATAGTGGCGCTCGACGAGTACGAGTCCCTCAAGGAGACGGCGTACC
AGCTCCGGAGTCCGGAGAACGCCCGTCGACTGCTGGCTTCGATCGACCGCCTGGAGAGCGGCGGCGGCACGGTGCAGGAG
CTTGCGGAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT178860 WP_003405863.1 NZ_CP063804:1244080-1244277 [Mycobacterium orygis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT178860 NZ_CP084357:c913338-913078 [Streptomyces sp. WA6-1-16]
GTGAAGTTCGTGTGGGACCAGTCGTCGTGGGACGACTACGTCTGGTGGCAGCACCAGGATCGCAAGATCCTCAAGAGGAT
CAACACGCTGCTCCAGGACATCGCCCGGAACGGCAACGAAGGAATGGGCAAGCCGGAGCCTCTGAAGCACGGCTTCCAGG
GGTACTGGTCCCGCCGTATCACCGACGAACACCGGCTCATCCACAAAGTGGTCGGCGACGAGGTCCGCATTGCCGCCTGC
CGCTACCACTACGGCCACTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07