Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1235324..1235955 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag RJtmp_RS05920 Protein ID WP_003405820.1
Coordinates 1235324..1235635 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag RJtmp_RS05925 Protein ID WP_003405836.1
Coordinates 1235635..1235955 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS05900 (RJtmp_001160) 1230806..1232230 - 1425 WP_003405805.1 class II fumarate hydratase -
RJtmp_RS05905 (RJtmp_001161) 1232261..1233349 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
RJtmp_RS05910 (RJtmp_001162) 1233405..1234049 + 645 WP_003915494.1 DUF4245 domain-containing protein -
RJtmp_RS05915 (RJtmp_001163) 1234056..1235212 - 1157 Protein_1165 AI-2E family transporter -
RJtmp_RS05920 (RJtmp_001164) 1235324..1235635 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
RJtmp_RS05925 (RJtmp_001165) 1235635..1235955 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
RJtmp_RS05930 (RJtmp_001166) 1235965..1237501 + 1537 Protein_1168 carboxylesterase/lipase family protein -
RJtmp_RS05935 (RJtmp_001167) 1237508..1238620 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
RJtmp_RS05940 (RJtmp_001168) 1238630..1238887 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
RJtmp_RS05945 (RJtmp_001169) 1238877..1240124 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
RJtmp_RS05950 (RJtmp_001170) 1240121..1240759 - 639 WP_003405849.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T178859 WP_003405820.1 NZ_CP063804:c1235635-1235324 [Mycobacterium orygis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T178859 NZ_CP084357:c913338-913078 [Streptomyces sp. WA6-1-16]
GTGAAGTTCGTGTGGGACCAGTCGTCGTGGGACGACTACGTCTGGTGGCAGCACCAGGATCGCAAGATCCTCAAGAGGAT
CAACACGCTGCTCCAGGACATCGCCCGGAACGGCAACGAAGGAATGGGCAAGCCGGAGCCTCTGAAGCACGGCTTCCAGG
GGTACTGGTCCCGCCGTATCACCGACGAACACCGGCTCATCCACAAAGTGGTCGGCGACGAGGTCCGCATTGCCGCCTGC
CGCTACCACTACGGCCACTGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT178859 WP_003405836.1 NZ_CP063804:c1235955-1235635 [Mycobacterium orygis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT178859 NZ_CP084357:c913586-913335 [Streptomyces sp. WA6-1-16]
ATGCGCACGATGACCTACACGGAGTCCCGCGCGAATTACGCCGAGACCCTGAGCGCGGTCGTGGACGACCGTGAAGAGGT
CGTGGTCACGCGGGCCGGCCACGAGCCCGTGGTGATAGTGGCGCTCGACGAGTACGAGTCCCTCAAGGAGACGGCGTACC
AGCTCCGGAGTCCGGAGAACGCCCGTCGACTGCTGGCTTCGATCGACCGCCTGGAGAGCGGCGGCGGCACGGTGCAGGAG
CTTGCGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References