Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 718566..719215 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag RJtmp_RS03300 Protein ID WP_003403236.1
Coordinates 718820..719215 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag RJtmp_RS03295 Protein ID WP_003403235.1
Coordinates 718566..718820 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS03270 (RJtmp_000649) 713691..713774 + 84 Protein_648 galactose-1-phosphate uridylyltransferase -
RJtmp_RS03275 (RJtmp_000650) 713793..714875 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
RJtmp_RS03280 (RJtmp_000651) 714872..715963 + 1092 WP_003403225.1 galactokinase -
RJtmp_RS03285 (RJtmp_000652) 716358..717515 + 1158 WP_003403229.1 hypothetical protein -
RJtmp_RS03290 (RJtmp_000653) 717526..718473 + 948 WP_003403232.1 DUF732 domain-containing protein -
RJtmp_RS03295 (RJtmp_000654) 718566..718820 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
RJtmp_RS03300 (RJtmp_000655) 718820..719215 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
RJtmp_RS03305 (RJtmp_000656) 719309..720049 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
RJtmp_RS03310 (RJtmp_000657) 720181..720441 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
RJtmp_RS03315 (RJtmp_000658) 720438..720845 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
RJtmp_RS03320 (RJtmp_000659) 720917..722068 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
RJtmp_RS03325 (RJtmp_000660) 722161..723888 - 1728 WP_003403250.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T178850 WP_003403236.1 NZ_CP063804:718820-719215 [Mycobacterium orygis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T178850 NZ_CP084343:c2124898-2124716 [Yersinia pestis]
GTGAAAAGTTCGGAGCTGATAAAACTGCTCGAAACAAATGGTTGGAAGTTGATGAGAGTTAAAGGCAGTCATCATCAATT
CAAACATCCTGATTTCGCGGCAGTCATAACAGTACCGCATCCCAAAAAGGATGTTAAACCGGGAACCCTCCGGCAAATTC
TGAAAGATGCAAACCTTAGTTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT178850 WP_003403235.1 NZ_CP063804:718566-718820 [Mycobacterium orygis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT178850 NZ_CP084343:c2124671-2124252 [Yersinia pestis]
ATGGCTATCTATCCCGCCTATGTTCACGTTGATAATGATGGCTCGGCAAGCGGTTATTTCCCCGATGTCAAAGGCTGTAT
TTTTGCCATCAATGCCGGGGAGGATTTATTTGCAGAAGCATCAAGCGCCCTGGATGCACACTTTGAAGCTTTGGTGTCTG
AAGGTATCGAAATTCCTGAAGCTCATGACATGCCTTACCACGTTTACCGTAACCCATGCGATTACGCTGATGGCGGACAG
TGGTATAACGTGAATATTGATATGTCGAAATATGATGGAAAAGTTGAACGAATTAACGTCACTCTTCCGCACCGGCTGAT
TCATCAAATCGATACTATTGTTAAAGTGCGTCCCGAGTATGCCAGCCGAAGCAATTTTCTAGCCGAAGCAGCACGTAAAG
AATTACAGAAATTGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References