Detailed information of TA system TA178848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 705399..706042
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: RJtmp_RS03215
• Protein ID: WP_003403187.1
• Coordinates: 705641..706042 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: RJtmp_RS03210
• Protein ID: WP_003403184.1
• Coordinates: 705399..705644 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS03175 (RJtmp_000630)	700679..701209	-	531	WP_003917320.1	two component system sensor kinase HK2	-
RJtmp_RS03180 (RJtmp_000631)	701193..701888	-	696	WP_003918099.1	two-component system response regulator TcrA	-
RJtmp_RS03185 (RJtmp_000632)	702011..702322	+	312	WP_003403164.1	hypothetical protein	-
RJtmp_RS03190 (RJtmp_000633)	702394..703344	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
RJtmp_RS03195 (RJtmp_000634)	703585..704169	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
RJtmp_RS03200 (RJtmp_000635)	704171..704881	+	711	Protein_634	IS607 family element RNA-guided endonuclease TnpB	-
RJtmp_RS03205 (RJtmp_000636)	704884..705354	+	471	WP_003898523.1	hypothetical protein	-
RJtmp_RS03210 (RJtmp_000637)	705399..705644	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RJtmp_RS03215 (RJtmp_000638)	705641..706042	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJtmp_RS03220 (RJtmp_04100)	706033..706212	+	180	Protein_638	hypothetical protein	-
RJtmp_RS03225 (RJtmp_000640)	706630..706827	-	198	WP_003403191.1	hypothetical protein	-
RJtmp_RS03230 (RJtmp_000641)	706907..708064	-	1158	WP_003403193.1	hypothetical protein	-
RJtmp_RS03235 (RJtmp_000642)	708116..708337	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
RJtmp_RS03240 (RJtmp_000643)	708479..709084	+	606	WP_003403205.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T178848 WP_003403187.1 NZ_CP063804:705641-706042 [Mycobacterium orygis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T178848 NZ_CP084343:c1123072-1122869 [Yersinia pestis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT178848 WP_003403184.1 NZ_CP063804:705399-705644 [Mycobacterium orygis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT178848 NZ_CP084343:c1123489-1123121 [Yersinia pestis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91