Detailed information of TA system TA178847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 699309..699955
• Replicon: chromosome
• Accession: NZ_CP063804
• Organism: Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: RJtmp_RS03160
• Protein ID: WP_003403137.1
• Coordinates: 699309..699722 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: RJtmp_RS03165
• Protein ID: WP_003403139.1
• Coordinates: 699719..699955 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJtmp_RS03140 (RJtmp_000623)	695392..696942	+	1551	WP_003403119.1	MlaD family protein	-
RJtmp_RS03145 (RJtmp_000624)	696994..697386	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
RJtmp_RS03150 (RJtmp_000625)	697383..697640	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
RJtmp_RS03155 (RJtmp_000626)	697823..699058	-	1236	WP_003403128.1	ATP-binding protein	-
RJtmp_RS03160 (RJtmp_000627)	699309..699722	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
RJtmp_RS03165 (RJtmp_000628)	699719..699955	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
RJtmp_RS03170 (RJtmp_000629)	700059..700565	-	507	WP_003403140.1	two component system sensor kinase HK1	-
RJtmp_RS03175 (RJtmp_000630)	700679..701209	-	531	WP_003917320.1	two component system sensor kinase HK2	-
RJtmp_RS03180 (RJtmp_000631)	701193..701888	-	696	WP_003918099.1	two-component system response regulator TcrA	-
RJtmp_RS03185 (RJtmp_000632)	702011..702322	+	312	WP_003403164.1	hypothetical protein	-
RJtmp_RS03190 (RJtmp_000633)	702394..703344	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
RJtmp_RS03195 (RJtmp_000634)	703585..704169	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
RJtmp_RS03200 (RJtmp_000635)	704171..704881	+	711	Protein_634	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T178847 WP_003403137.1 NZ_CP063804:c699722-699309 [Mycobacterium orygis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T178847 NZ_CP084342:7810-8151 [Yersinia pestis]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT178847 WP_003403139.1 NZ_CP063804:c699955-699719 [Mycobacterium orygis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT178847 NZ_CP084342:8151-8450 [Yersinia pestis]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89