Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696994..697640 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag RJtmp_RS03145 Protein ID WP_003403122.1
Coordinates 696994..697386 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag RJtmp_RS03150 Protein ID WP_003403125.1
Coordinates 697383..697640 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS03130 (RJtmp_000621) 692653..694182 + 1530 WP_031711997.1 virulence factor Mce family protein -
RJtmp_RS03135 (RJtmp_000622) 694245..695387 + 1143 WP_003911253.1 virulence factor Mce family protein -
RJtmp_RS03140 (RJtmp_000623) 695392..696942 + 1551 WP_003403119.1 MlaD family protein -
RJtmp_RS03145 (RJtmp_000624) 696994..697386 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
RJtmp_RS03150 (RJtmp_000625) 697383..697640 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
RJtmp_RS03155 (RJtmp_000626) 697823..699058 - 1236 WP_003403128.1 ATP-binding protein -
RJtmp_RS03160 (RJtmp_000627) 699309..699722 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
RJtmp_RS03165 (RJtmp_000628) 699719..699955 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
RJtmp_RS03170 (RJtmp_000629) 700059..700565 - 507 WP_003403140.1 two component system sensor kinase HK1 -
RJtmp_RS03175 (RJtmp_000630) 700679..701209 - 531 WP_003917320.1 two component system sensor kinase HK2 -
RJtmp_RS03180 (RJtmp_000631) 701193..701888 - 696 WP_003918099.1 two-component system response regulator TcrA -
RJtmp_RS03185 (RJtmp_000632) 702011..702322 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T178846 WP_003403122.1 NZ_CP063804:c697386-696994 [Mycobacterium orygis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T178846 NZ_CP084341:c33967-33668 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT178846 WP_003403125.1 NZ_CP063804:c697640-697383 [Mycobacterium orygis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT178846 NZ_CP084341:c34202-33960 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References