Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3305602..3306197 Replicon chromosome
Accession NZ_CP063774
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag INT78_RS15675 Protein ID WP_000239579.1
Coordinates 3305847..3306197 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag INT78_RS15670 Protein ID WP_001223208.1
Coordinates 3305602..3305853 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INT78_RS15660 3301267..3305046 + 3780 WP_000060945.1 autotransporter assembly complex protein TamB -
INT78_RS15665 3305049..3305390 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
INT78_RS15670 3305602..3305853 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
INT78_RS15675 3305847..3306197 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
INT78_RS15680 3306277..3306807 - 531 WP_000055075.1 inorganic diphosphatase -
INT78_RS15685 3307117..3308073 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
INT78_RS15690 3308213..3309715 + 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
INT78_RS15695 3309729..3310751 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T178804 WP_000239579.1 NZ_CP063774:3305847-3306197 [Escherichia coli O25b:H4-ST131]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T178804 NZ_CP084301:c21828-21538 [Acinetobacter pseudolwoffii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TTGCTAAAGAAGCAGGGATTGGGCGTGAGGCACTCTACAAAGCCCTACGTCATGATTCAGCACCACGTTTTGATACCATT
AATCGTGTAGTGAATGCTTTAGGCTTAAAACTCACAGTACAACATGCCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT178804 WP_001223208.1 NZ_CP063774:3305602-3305853 [Escherichia coli O25b:H4-ST131]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT178804 NZ_CP084301:c22126-21830 [Acinetobacter pseudolwoffii]
ATGATTGAAATTAAACGTCTGCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATTCGCCTGAA
TCGTAGATTAGATAAGGTTCAACGTGGAAATTGGGGAGACATTAAGCCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTGCTGGATGGAGAATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGAGGAGATAAATCG
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACATTGGAGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References