Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2388640..2389440 Replicon chromosome
Accession NZ_CP063774
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag INT78_RS11455 Protein ID WP_000342452.1
Coordinates 2388640..2389167 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag INT78_RS11460 Protein ID WP_001277107.1
Coordinates 2389174..2389440 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INT78_RS11430 2383716..2384483 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
INT78_RS11435 2384480..2385757 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
INT78_RS11440 2385754..2386680 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
INT78_RS11445 2386728..2387837 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
INT78_RS11450 2388260..2388643 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
INT78_RS11455 2388640..2389167 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
INT78_RS11460 2389174..2389440 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
INT78_RS11465 2389589..2390692 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
INT78_RS11470 2390963..2391817 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
INT78_RS11475 2392062..2393120 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
INT78_RS11480 2393113..2393781 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T178801 WP_000342452.1 NZ_CP063774:c2389167-2388640 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T178801 NZ_CP084299:c3822-3526 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTACCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGCATTCGCTTGAA
TCGCCGATTAGATAAGGTTCAACGTGGGAACTGGGGAGACATTAAACCTCTCCAAGATGGTGTTTGGGAAATGAGAGAGT
TCTTCGGTTCAGGATGGAGGATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGAGGTGGTGATAAATCT
ACACAGCAAAAGGATATAGAACGAGCTGTTGATTTATCAAAATCTTTAGAGGATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT178801 WP_001277107.1 NZ_CP063774:c2389440-2389174 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT178801 NZ_CP084299:c3522-3232 [Acinetobacter baumannii]
ATGGTTAAAATAGCTGATCTACCTAGCTTTGACATGGCAGAAGCACTTAAGAGTGAAGAAGATATTGTAATGTATCTTAA
TATGGTTCTTGAAGAGAATGACCCTGCTGAACTAGCTCATGCACTTGGTGTAATTGCTAAAGCTCGTGGTATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGACGTGAAGCACTTTACAAAGCACTCCGTCAAGATTCAGCACCTCGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGTCTAAAACTAACTGTGCAGAATGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References