Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 234363..235951 Replicon chromosome
Accession NZ_CP063774
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag INT78_RS01080 Protein ID WP_001125411.1
Coordinates 234363..235685 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag INT78_RS01085 Protein ID WP_001296076.1
Coordinates 235685..235951 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INT78_RS01070 231817..232527 - 711 WP_001195176.1 fimbria/pilus periplasmic chaperone -
INT78_RS01075 232889..233452 - 564 WP_001296074.1 type 1 fimbrial major subunit FimA -
INT78_RS01080 234363..235685 - 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
INT78_RS01085 235685..235951 - 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
INT78_RS01090 236259..237017 + 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
INT78_RS01095 237021..237935 - 915 WP_001296077.1 bestrophin family protein -
INT78_RS01100 238132..239583 - 1452 WP_000854638.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T178786 WP_001125411.1 NZ_CP063774:c235685-234363 [Escherichia coli O25b:H4-ST131]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T178786 NZ_CP084290:c4751012-4750638 [Citrobacter werkmanii]
ATGGTCAAAGGGGCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTGGCGAT
AGAAACATGGCCGCCACAGAACGCAATCAGTCTCATTACATGGATGGAGGTGATGGTAGGCGCAAAAAAATACCATCAGG
AGGCCCGTACTCGCATTGCGCTAGGCGCTTTTAACATTATTGGTGTTTCACAGGATATTGCTGAGCGAAGCGTGAGTATC
AGGCAGGAGCAGGGGATGAAATTGCCGGATGCCATTATTTTAGCTACTGCGCAGATTCATCATCTGACACTGGTTACGCG
TAATACCAAAGATTTTGCAAGCATATCCGGTGTGGTCACACCGTATACGTTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT178786 WP_001296076.1 NZ_CP063774:c235951-235685 [Escherichia coli O25b:H4-ST131]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT178786 NZ_CP084290:c4751254-4751012 [Citrobacter werkmanii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAAACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References