Detailed information of TA system TA178784

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4408840..4409564
• Replicon: chromosome
• Accession: NZ_CP063773
• Organism: Pectobacterium carotovorum subsp. carotovorum PCCS1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: IQ281_RS19755
• Protein ID: WP_194430412.1
• Coordinates: 4408840..4409160 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: IQ281_RS19760
• Protein ID: WP_194430413.1
• Coordinates: 4409229..4409564 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ281_RS19725	4404133..4405548	+	1416	WP_194430408.1	diguanylate cyclase	-
IQ281_RS19730	4405821..4406039	+	219	WP_181848682.1	KTSC domain-containing protein	-
IQ281_RS19735	4406267..4406623	+	357	WP_194430409.1	hypothetical protein	-
IQ281_RS19740	4407161..4407565	-	405	WP_194430410.1	tRNA(fMet)-specific endonuclease VapC	-
IQ281_RS19745	4407565..4407795	-	231	WP_005974492.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IQ281_RS19750	4407889..4408722	-	834	WP_194430411.1	DUF4942 domain-containing protein	-
IQ281_RS19755	4408840..4409160	-	321	WP_194430412.1	TA system toxin CbtA family protein	Toxin
IQ281_RS19760	4409229..4409564	-	336	WP_194430413.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQ281_RS19765	4409604..4410077	-	474	WP_194430414.1	DNA repair protein RadC	-
IQ281_RS19770	4410200..4410550	-	351	WP_194430415.1	hypothetical protein	-
IQ281_RS19775	4410608..4411441	-	834	WP_194430416.1	hypothetical protein	-
IQ281_RS19780	4411588..4411746	-	159	WP_155242703.1	hypothetical protein	-
IQ281_RS19785	4411930..4412817	-	888	WP_194430417.1	50S ribosome-binding GTPase	-
IQ281_RS19790	4412913..4413944	-	1032	WP_194430418.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheB / cheR / cheD / cheW / cheA / motB / motA / flhC / flhD	4390040..4437151	47111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12079.83 Da
• Isoelectric Point: 5.0890

>T178784 WP_194430412.1 NZ_CP063773:c4409160-4408840 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MQTSPAIPPREDNLCPSPITVRQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGITLVNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T178784 NZ_CP084290:4463675-4464052 [Citrobacter werkmanii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12349.10 Da
• Isoelectric Point: 7.2404

>AT178784 WP_194430413.1 NZ_CP063773:c4409564-4409229 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MPSSDTPEWGLKCAITPRFGARLVQEGNRLHYLADRASVVGTFSKTEARHLEHVFPELIKQLEQKLRTGKLNPCQQGCIV
LHCNEWTCEADTLGSVGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT178784 NZ_CP084290:4463259-4463585 [Citrobacter werkmanii]
ATGAGTAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATAAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCAACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA