Detailed information of TA system TA178780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2370620..2371404
• Replicon: chromosome
• Accession: NZ_CP063773
• Organism: Pectobacterium carotovorum subsp. carotovorum PCCS1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: IQ281_RS10645
• Protein ID: WP_194431685.1
• Coordinates: 2370910..2371404 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: IQ281_RS10640
• Protein ID: WP_194431684.1
• Coordinates: 2370620..2370913 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ281_RS10620	2366425..2367444	-	1020	WP_194431681.1	alpha/beta hydrolase	-
IQ281_RS10625	2367605..2368027	+	423	WP_039283843.1	MarR family transcriptional regulator	-
IQ281_RS10630	2368152..2369339	-	1188	WP_194431682.1	MFS transporter	-
IQ281_RS10635	2369455..2370411	+	957	WP_194431683.1	AraC family transcriptional regulator	-
IQ281_RS10640	2370620..2370913	+	294	WP_194431684.1	DUF1778 domain-containing protein	Antitoxin
IQ281_RS10645	2370910..2371404	+	495	WP_194431685.1	GNAT family N-acetyltransferase	Toxin
IQ281_RS10650	2371630..2372628	-	999	WP_194431686.1	hypothetical protein	-
IQ281_RS10655	2372796..2373107	+	312	WP_194431807.1	hypothetical protein	-
IQ281_RS10660	2373003..2373262	-	260	Protein_2062	type II toxin-antitoxin system toxin CcdB	-
IQ281_RS10665	2373310..2373528	-	219	WP_194431687.1	type II toxin-antitoxin system antitoxin CcdA	-
IQ281_RS10670	2373543..2373791	-	249	WP_194431688.1	helix-turn-helix domain-containing protein	-
IQ281_RS10675	2373877..2374131	-	255	WP_039285388.1	helix-turn-helix domain-containing protein	-
IQ281_RS10680	2374235..2374477	+	243	WP_194431689.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2355567..2374477	18910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17702.57 Da
• Isoelectric Point: 7.7788

>T178780 WP_194431685.1 NZ_CP063773:2370910-2371404 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T178780 NZ_CP063773:2370910-2371404 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
ATGATTTCCGCCCCTGAGCCACTCCGCGCCGAACATGTACTATCTTCATTCTGTTGCGGCGTGGAGTCCATGGATAACTG
GCTAAAACAACGGGCGATGAAAAATCAGGTCACTGGAGCATCACGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCCGTGGCAACGAATGCCGCTCCTGGACGTTTTCGTCGCAATATGCCCGATCCTATT
CCGGTCGTGGTACTGGGTCGTCTGGCGGTGGATAAATCATTGCATGGTCAGGGCGTTGGCCGGGCTTTGGTACGTGATGC
TGGGCTAAGAGTGATTCAAGTGGCGGATACCATCGGTATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCACGGG
AATTCTACCTGCGGGTGGGGTTTGAGCCTTCACCGATTGATCCGATGATGTTGATGGTAACGTTGGGGGATTTGGTGGGT
AGTTTGTCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.58 Da
• Isoelectric Point: 10.1968

>AT178780 WP_194431684.1 NZ_CP063773:2370620-2370913 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEVLIDQRIIMADPDAYQEFLTRLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT178780 NZ_CP063773:2370620-2370913 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGTGAAACATTGAATTTGCGCATCAAGCCCGCCGAACGCGATCTGATTGA
TCGTGCAGCAAAGGCTAGGGGAAAAAATCGCACTGATTTTGTCCTTGAGGCTGCGCGTGCCGCCGCAGAGGAAGTGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTACTCGCTTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGAAAAACCATGCAAACGCCAGCACCGTGGGAACAGAAAAAATGA