Detailed information of TA system TA178777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1854312..1855956
• Replicon: chromosome
• Accession: NZ_CP063773
• Organism: Pectobacterium carotovorum subsp. carotovorum PCCS1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IQ281_RS08435
• Protein ID: WP_194431491.1
• Coordinates: 1854622..1855956 (+)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IQ281_RS08430
• Protein ID: WP_194431490.1
• Coordinates: 1854312..1854617 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ281_RS08405	1849844..1850959	+	1116	WP_181829932.1	LPS export ABC transporter permease LptF	-
IQ281_RS08410	1850959..1852035	+	1077	WP_181829931.1	LPS export ABC transporter permease LptG	-
IQ281_RS08420	1852569..1853825	+	1257	WP_194431750.1	tyrosine-type recombinase/integrase	-
IQ281_RS08425	1853844..1854005	+	162	WP_194431489.1	CopG family transcriptional regulator	-
IQ281_RS08430	1854312..1854617	+	306	WP_194431490.1	helix-turn-helix transcriptional regulator	Antitoxin
IQ281_RS08435	1854622..1855956	+	1335	WP_194431491.1	type II toxin-antitoxin system HipA family toxin	Toxin
IQ281_RS08440	1856231..1858267	-	2037	WP_194431492.1	AAA family ATPase	-
IQ281_RS08445	1858260..1858793	-	534	WP_194431751.1	ash family protein	-
IQ281_RS08450	1858793..1858993	-	201	WP_005971237.1	AlpA family transcriptional regulator	-
IQ281_RS08455	1858996..1859874	-	879	WP_194431493.1	integrase domain-containing protein	-
IQ281_RS08460	1860419..1860670	-	252	WP_180778919.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1854312..1865187	10875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 50642.76 Da
• Isoelectric Point: 6.1895

>T178777 WP_194431491.1 NZ_CP063773:1854622-1855956 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MPRTQQRLAIWMNGIQVGFWEKTRGEDRLQYLPEWIADEQGRPLSLSLLFTPGNQLWRGNVVRDYFDNLLPDSEGIRRRL
ATRYHADSLEPFDLLAELGRDCVGAIQLLNGNEEPTDLFSVKYRPLCDAEIAAILRNTTAALLPGRQDDTDDLRLSIAGA
QEKTALLWHEGQWCLPEGSTPTTHIFKLPLGLVGNMQADMSTSVENEWLCSLILKEYGIPVAKTQIAQFEEQKALVVERF
DRRWSSDRQWVIRLPQEDMCQALGVSPLRKYQSDGGPGISDIMTILSHSEQAGQDKAQFFRAQIIFWLIAATDGHAKNFS
IAIEPQGRYHLTPLYDVLSAWPVIGPCNNQISWQKCKLAMAVRGSSNYYHLYRIKRRHWVNHGELTGLGRRQVEAMMDDI
ISRTPEVIERVSSLLPESFPPELAECVFDGMRQQCGRLQELHRK

Nucleotide

• Length: 1335 bp

>T178777 NZ_CP084290:282300-282575 [Citrobacter werkmanii]
ATGAAGGAAAAGGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCGCCTACGCCGCAGGG
AATCAAGTGGTCGGAAATCGAGTCACTGATTAAGGCATTAGGTGGCGAAATCAAAGAAGGGCGAGGTTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATCGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11257.00 Da
• Isoelectric Point: 10.3119

>AT178777 WP_194431490.1 NZ_CP063773:1854312-1854617 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MNTVYPLKTLNQLRPLVIGFRKAKGLTQKDVSERLGVTQQTYARLEANPGSASIERLFKVLTVLGVEVVLSSGPNVHFSM
SSSTMEEPEKPIDSPARREKW

Nucleotide

• Length: 306 bp

>AT178777 NZ_CP084290:282572-282931 [Citrobacter werkmanii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCTGGGCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTCCGCGGTAAGTTCCTGGGTTTATCAGGCTATTGTGATTTTGTTGCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATTTCCTTACGCGAGTATCTGGATGATTGCTCTGCCGCAGGGATAGAGCCCTATGCTCACCCGGAAAAGCTGAAGACA
TTTACGCTACGCTACCCAGAGTCATTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTGAATACCTG
GATTATCGAAACGCTTAGTAAAAGCCTGAAACAGGCATAG