Detailed information of TA system TA178771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1125351..1125968
• Replicon: chromosome
• Accession: NZ_CP063773
• Organism: Pectobacterium carotovorum subsp. carotovorum PCCS1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C6DHN3
• Locus tag: IQ281_RS05145
• Protein ID: WP_010281507.1
• Coordinates: 1125351..1125530 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IQ281_RS05150
• Protein ID: WP_181847422.1
• Coordinates: 1125558..1125968 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ281_RS05120	1120586..1121746	-	1161	WP_012772912.1	cystathionine gamma-synthase	-
IQ281_RS05125	1121934..1122251	+	318	WP_005973355.1	met regulon transcriptional regulator MetJ	-
IQ281_RS05130	1122467..1123264	+	798	WP_194431280.1	ABC transporter ATP-binding protein	-
IQ281_RS05135	1123274..1124236	+	963	WP_194431281.1	ABC transporter substrate-binding protein	-
IQ281_RS05140	1124233..1125253	+	1021	Protein_990	iron ABC transporter permease	-
IQ281_RS05145	1125351..1125530	+	180	WP_010281507.1	type II toxin-antitoxin system HicA family toxin	Toxin
IQ281_RS05150	1125558..1125968	+	411	WP_181847422.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IQ281_RS05155	1126110..1126325	-	216	WP_012772907.1	50S ribosomal protein L31	-
IQ281_RS05160	1126640..1128838	+	2199	WP_194431282.1	primosomal protein N'	-
IQ281_RS05165	1129115..1130158	+	1044	WP_194431283.1	DNA-binding transcriptional regulator CytR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6560.69 Da
• Isoelectric Point: 10.7838

>T178771 WP_010281507.1 NZ_CP063773:1125351-1125530 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T178771 NZ_CP084283:c153691-153407 [Caballeronia zhejiangensis]
ATGCTCGTTGATTGGCGGCCGCGCGCACGGAGACAACTCAGGGAAATCTACGAATACATTGAAGAGCGTGATCCGCAAGC
GGCCGACAATCTACTTTCGATGAACAGGGAAGCAGTCCGGACGTTAGCGGATGTTCCATACATCTATCGTCACGGAAGGG
TCTCGAATACCAGAGAGATGGTGGTTCATCCGAATTACTTGGTGGTTTACCGTGCGACGGACCGCGTCACCGTACTGGAC
TCGCTGCATGCACGGCGGCGGTATCCGAGCGACAAGACGCGCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14591.49 Da
• Isoelectric Point: 4.4493

>AT178771 WP_181847422.1 NZ_CP063773:1125558-1125968 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLVEMGQDIPTVSTVGQLAKRSEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Nucleotide

• Length: 411 bp

>AT178771 NZ_CP084283:c153872-153681 [Caballeronia zhejiangensis]
ATGGACAAACGACTCGATCCGCTTATTTCCGAACTCGATTCGCTCGAAGAGGCTGAACTGTATGACGCCTGGTTCCGCGC
GGAGGTAGAAGCGTCACTTGCGGACCCGGAGCCGTCGATTCCCAACGATCAAGTATTCGCGGAAATGGATGCGCTCGTGG
CGGCCAAGCGAAAGGCACGGAATGCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7W2I1S0