Detailed information of TA system TA178761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3586421..3587338
• Replicon: chromosome
• Accession: NZ_CP063768
• Organism: Bacillus velezensis strain KMU01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: IM712_RS17320
• Protein ID: WP_057079950.1
• Coordinates: 3586421..3587167 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: IM712_RS17325
• Protein ID: WP_003154807.1
• Coordinates: 3587168..3587338 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM712_RS17300	3581813..3582763	-	951	WP_077722102.1	ring-cleaving dioxygenase	-
IM712_RS17305	3583086..3584402	+	1317	WP_003154801.1	amino acid permease	-
IM712_RS17310	3584688..3585305	+	618	WP_003154804.1	DUF47 domain-containing protein	-
IM712_RS17315	3585318..3586316	+	999	WP_003154805.1	inorganic phosphate transporter	-
IM712_RS17320	3586421..3587167	+	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IM712_RS17325	3587168..3587338	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IM712_RS17330	3587595..3588473	-	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
IM712_RS17335	3588487..3588750	-	264	WP_003154813.1	phage holin	-
IM712_RS17340	3588764..3589027	-	264	WP_003154815.1	hemolysin XhlA family protein	-
IM712_RS17345	3589079..3589840	-	762	WP_070081683.1	phage portal protein	-
IM712_RS17350	3589897..3590094	-	198	WP_070081682.1	XkdX family protein	-
IM712_RS17355	3590099..3590524	-	426	WP_077722101.1	hypothetical protein	-
IM712_RS17360	3590537..3592126	-	1590	WP_077722100.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T178761 WP_057079950.1 NZ_CP063768:3586421-3587167 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T178761 NZ_CP084248:2082158-2082577 [Enterococcus lactis]
GTGATTTCTGAAGGACTTTATTTTGAAGCTGCAGACCTTGCAAATGAACTCTCAGAAGAAGTTAGTTATTACTGTTCAAA
GAAAATGGAACATGTAAAATGCTACGACATTGAGAATTATGTGAATGAAGTAGAAGATGTAGAATTCATCGATTACGACT
TCAAAGATAACTTAAGTAAGAAAATGCTTGGTTCCATTACAAAAGTTCATGGCGAAATCATCATTACCACCAATCAACAT
TTAATGCTTGAACGCAAAAATTTTACAAAAATGCATGAGATCATCCATTATTATAAGGACGTCCCCTATCTTGGAGAATC
TCATGCATTTTCTGATATGATTTTAGAAGATACTTATTTGCCAGAAGACTATAAAAAAACTTCGAGCAAATATAGGTGCC
AGTATCCTCATGGCGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT178761 WP_003154807.1 NZ_CP063768:3587168-3587338 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT178761 NZ_CP084248:2081880-2082134 [Enterococcus lactis]
TTGTCAAATTATCAGCTAGTCACAGTAATAGCAGATTTCTTCAATGTTTCTACAGATTTTCTACTAAATAGAACCTCTGA
TCCGACTCCTCCAAAACGTAGCGAAGAAGATCAGCTTGGAGACAGTATTCTTTCTCACTTCAGATTGAATACTGCAGATA
TGGAAGTAGAAGACATTGAAGAATTGGAAGAAGAATTAAATGAATATATGGACTTTTTAGTTCAACGTGCAAAAGCAAAG
AAAAATAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14