178760

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 389757..390394 Replicon chromosome
Accession NZ_CP063768
Organism Bacillus velezensis strain KMU01

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IM712_RS01995 Protein ID WP_003156187.1
Coordinates 389757..390107 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag IM712_RS02000 Protein ID WP_003156188.1
Coordinates 390113..390394 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM712_RS01955 384797..385399 - 603 WP_003156170.1 SpoIIE family protein phosphatase -
IM712_RS01960 385399..386187 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
IM712_RS01965 386153..386635 - 483 WP_003156173.1 anti-sigma B factor RsbW -
IM712_RS01970 386632..386961 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
IM712_RS01975 387025..388032 - 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
IM712_RS01980 388044..388445 - 402 WP_003156178.1 anti-sigma regulatory factor -
IM712_RS01985 388448..388813 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
IM712_RS01990 388818..389639 - 822 WP_003156182.1 STAS domain-containing protein -
IM712_RS01995 389757..390107 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IM712_RS02000 390113..390394 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IM712_RS02005 390514..391683 - 1170 WP_077721837.1 alanine racemase -
IM712_RS02010 391800..392807 - 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
IM712_RS02015 392972..393337 - 366 WP_003156192.1 holo-ACP synthase -
IM712_RS02020 393430..394029 + 600 WP_077721836.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T178760 WP_003156187.1 NZ_CP063768:c390107-389757 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T178760 NZ_CP084248:c2079253-2078987 [Enterococcus lactis]
ATGACCTACCGTTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAGCATCAAGCGACGCTGATCGT
TCGATGGCTGTATCAACATATTGATGGTATTGACGATCCGAGAAAATTCGGAAAAGGATTGACCGCTAATCGTTCAGGAC
AATGGCGTTATCGAATTGGCAAATATCGTGTGTTGGTAGAGATAGAAGATAATCAAATAGTTGTTATCGCTATTCAAGTG
GGACACCGAAAAAATGTCTATGATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT178760 WP_003156188.1 NZ_CP063768:c390394-390113 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT178760 NZ_CP084248:c2079480-2079250 [Enterococcus lactis]
ATGGCTATGATTACGGTTCGTGTGAGTGATGCCGAAAAAGAATGGCTTTCTTATATGGCGGAATTTTATGGGATTACTTT
ATCTGAGCTTCTAAAAACCTATTCGATGGAACAACTAGAAGATGAATACGATCGTCAAACGGCTGAAATTGCCCATAAAC
ATTGGGTAGAAAGTGGAAAAACAACAGTATCCATGGAAGAAATCCTTCGTGAATTTGGAGGGTTGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References