Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 1128195..1128688 Replicon chromosome
Accession NZ_CP063766
Organism Enterococcus lactis strain IDCC 2105

Toxin (Protein)


Gene name relE Uniprot ID J6KIM0
Locus tag IPZ63_RS05465 Protein ID WP_002400299.1
Coordinates 1128422..1128688 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4Y3JLV3
Locus tag IPZ63_RS05460 Protein ID WP_025478324.1
Coordinates 1128195..1128425 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPZ63_RS05435 (IPZ63_05415) 1124444..1125211 + 768 WP_002400309.1 MurR/RpiR family transcriptional regulator -
IPZ63_RS05440 (IPZ63_05420) 1125212..1125655 + 444 WP_002400306.1 hypothetical protein -
IPZ63_RS05445 (IPZ63_05425) 1125826..1126454 - 629 Protein_1081 glutamine amidotransferase -
IPZ63_RS05450 1127040..1127540 + 501 WP_002400304.1 hypothetical protein -
IPZ63_RS05455 (IPZ63_05435) 1127549..1127704 + 156 WP_002400302.1 hypothetical protein -
IPZ63_RS05460 (IPZ63_05440) 1128195..1128425 + 231 WP_025478324.1 DUF6290 family protein Antitoxin
IPZ63_RS05465 (IPZ63_05445) 1128422..1128688 + 267 WP_002400299.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IPZ63_RS05470 (IPZ63_05450) 1128939..1129167 + 229 Protein_1086 hypothetical protein -
IPZ63_RS05475 (IPZ63_05455) 1129183..1130349 + 1167 WP_002314829.1 radical SAM family heme chaperone HemW -
IPZ63_RS05480 (IPZ63_05460) 1130583..1131626 + 1044 WP_002294449.1 heat-inducible transcriptional repressor HrcA -
IPZ63_RS05485 (IPZ63_05465) 1131720..1132283 + 564 WP_002400298.1 nucleotide exchange factor GrpE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-87)

Antitoxin

Protein of unknown function DUF6290

(1-71)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10642.41 Da        Isoelectric Point: 10.4982

>T178759 WP_002400299.1 NZ_CP063766:1128422-1128688 [Enterococcus lactis]
MTYRLEFTPEIQKQLRKMDKHQAMLIVRWLYQHIDGIDDPRKFGKGLTANRSGQWRYRIGKYRVLVEIEDKQLVVIAIQV
GHRRNIYD

Download         Length: 267 bp

>T178759 NZ_CP084247:c231267-230995 [Enterococcus lactis]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8790.97 Da        Isoelectric Point: 4.4723

>AT178759 WP_025478324.1 NZ_CP063766:1128195-1128425 [Enterococcus lactis]
MAMITVRVSDAEKEWLSYMAKFYGITLSELLKTYSMEQLEDEYDRQTAEIAHKHWVESGKTTVSMEEILHEFGGLG

Download         Length: 231 bp

>AT178759 NZ_CP084247:c231530-231267 [Enterococcus lactis]
ATGGCAAAAACTGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y3JQ03

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y3JLV3

References