Detailed information of TA system TA178751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 144014..144684
• Replicon: plasmid pFRPDR_1
• Accession: NZ_CP063760
• Organism: Klebsiella pneumoniae strain FRPDR

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: IM727_RS28270
• Protein ID: WP_004213072.1
• Coordinates: 144014..144457 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: IM727_RS28275
• Protein ID: WP_004213073.1
• Coordinates: 144454..144684 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM727_RS28235	139425..139700	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
IM727_RS28240	139763..140254	+	492	WP_011251327.1	DM13 domain-containing protein	-
IM727_RS28245	140303..141223	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
IM727_RS28250	141314..141720	+	407	Protein_149	GAF domain-containing protein	-
IM727_RS28255	142235..142870	-	636	Protein_150	response regulator	-
IM727_RS28260	143287..143580	+	294	Protein_151	transposase	-
IM727_RS28265	143584..144000	+	417	Protein_152	restriction endonuclease	-
IM727_RS28270	144014..144457	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
IM727_RS28275	144454..144684	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IM727_RS28280	145292..146425	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
IM727_RS28285	146441..146734	+	294	WP_004213076.1	hypothetical protein	-
IM727_RS28290	146724..146930	-	207	WP_004213077.1	hypothetical protein	-
IM727_RS28295	147282..147572	+	291	WP_004213078.1	hypothetical protein	-
IM727_RS28300	147562..148461	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..219933	219933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T178751 WP_004213072.1 NZ_CP063760:c144457-144014 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T178751 NZ_CP084241:c3819082-3818765 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT178751 WP_004213073.1 NZ_CP063760:c144684-144454 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT178751 NZ_CP084241:c3818772-3818485 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620