178748

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5479324..5479949 Replicon chromosome
Accession NZ_CP063759
Organism Klebsiella pneumoniae strain FRPDR

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IM727_RS27140 Protein ID WP_002882817.1
Coordinates 5479324..5479707 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IM727_RS27145 Protein ID WP_004150355.1
Coordinates 5479707..5479949 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM727_RS27125 5476690..5477592 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IM727_RS27130 5477589..5478224 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IM727_RS27135 5478221..5479150 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IM727_RS27140 5479324..5479707 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IM727_RS27145 5479707..5479949 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IM727_RS27150 5480154..5481071 + 918 WP_002882812.1 alpha/beta hydrolase -
IM727_RS27155 5481085..5482026 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IM727_RS27160 5482071..5482508 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IM727_RS27165 5482505..5483365 - 861 WP_002882807.1 virulence factor BrkB family protein -
IM727_RS27170 5483359..5483958 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T178748 WP_002882817.1 NZ_CP063759:c5479707-5479324 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T178748 NZ_CP084241:797459-797869 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT178748 WP_004150355.1 NZ_CP063759:c5479949-5479707 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT178748 NZ_CP084241:797212-797478 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References