Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4895805..4896615 Replicon chromosome
Accession NZ_CP063759
Organism Klebsiella pneumoniae strain FRPDR

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag IM727_RS24355 Protein ID WP_002887280.1
Coordinates 4895805..4896338 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IM727_RS24360 Protein ID WP_002887278.1
Coordinates 4896349..4896615 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM727_RS24350 4894636..4895757 + 1122 WP_002887282.1 cupin domain-containing protein -
IM727_RS24355 4895805..4896338 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
IM727_RS24360 4896349..4896615 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
IM727_RS24365 4896718..4898151 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
IM727_RS24370 4898141..4898824 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IM727_RS24375 4898996..4900381 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
IM727_RS24380 4900399..4900743 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T178745 WP_002887280.1 NZ_CP063759:c4896338-4895805 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T178745 NZ_CP084235:970984-971133 [Staphylococcus haemolyticus]
ATGACTAAAAATAAAGATTCAGAATTAAATTATCATGAGGAAGAAAATGCGATGGTTCAAGATTTAGATGACTTGAAAAC
ATTGGGCAAAGAAATGGAACAAATTTCTGAAGAAAACGACGAGGACAGGTTGAATCAATCGCATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT178745 WP_002887278.1 NZ_CP063759:c4896615-4896349 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT178745 NZ_CP084235:970362-970961 [Staphylococcus haemolyticus]
ATGGCAATGAACTTTAAAGTATTTAATGATGTAGAACATGTAGCTGAGTACACAGCAGATATCATTAGAAAGCAATTTAA
TAACAACCCTACAACTATTGCGGGAATTCATTTAACAAAGGATGCTGCACCTGTTTTAGATGAGTTAAAGAAAGATGTAG
ATCATAATGCGGTTGATTTTAGTCAAGTAAATATCTTAGATTATGACGATAATCGCTCATACTATGAAGCATTAGGTGTA
CCTGCTAGTCAAATTTATCCGATTAATCTTGACGATGATGCAGAATCTTTAATTGATGACAAAATTAAAACTAAAGAAAA
TAAAGGTAAATTAATTTTACAAGTTACTTCTATTGATGAATCAGGTAGTTTAAATGTAAATGTAAGACAAGGATTATTAA
AAGCGCGTGAAGTAGTATTAGTTGTGACTGGTGCTAATAAACGTGAAGTTGTTAAAAAACTTTATGAAGAAAATGGTAAA
TCTAGCTTTGAACCAGCTGATTTAAAAGCTCATAGAATGGTAACAGTTGTTTTAGATAGAGCAGCTGCAGAAGGTTTACC
TGAAGATGTTAAAGAATATTTCACTGCTAGATTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References